Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore, 575018, India.
Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, UK.
Arch Microbiol. 2020 Aug;202(6):1571-1579. doi: 10.1007/s00203-020-01848-5. Epub 2020 Mar 12.
Enterobacter cloacae is normally considered to be an opportunistic human pathogen. Here, we report on the whole-genome sequence of an endophytic E. cloacae, strain "Ghats1", isolated from leaves of the medicinal plant Coscinium fenestratum Gaertn. Functional analysis of the Ghats1 genome revealed an enrichment for genes involved in the uptake and exchange of nutrients, for chemotaxis and for plant colonization. Unexpectedly though, there were no ORFs belonging to the "virulence factors and antibiotic resistance". Moreover, the presence of hydrolytic enzymes and motility functions reveals the characteristics of an endophyte lifestyle of a bacterium that can colonize and adapt to plant environment. These results provide a better understanding of an endophytic lifestyle through plant-microbe interaction, which can be further exploited as a biocontrol agent.
阴沟肠杆菌通常被认为是一种机会性病原体。在这里，我们报告了一株内生阴沟肠杆菌菌株“Ghats1”的全基因组序列，该菌株从药用植物海风藤的叶片中分离得到。Ghats1 基因组的功能分析显示，其富含参与营养物质摄取和交换、趋化性和植物定殖的基因。然而，出人意料的是，没有属于“毒力因子和抗生素耐药性”的 ORFs。此外，水解酶和运动功能的存在揭示了一种能够定殖和适应植物环境的细菌内生生活方式的特征。这些结果通过植物-微生物相互作用更好地理解了内生生活方式，这可以进一步被开发为一种生物防治剂。
