Sioud M, Baldacci G, Forterre P, de Recondo A M
Laboratoire de Biologie Moléculaire de la Replication, CNRS-IRSC, Villejuif, France.
Nucleic Acids Res. 1988 Aug 25;16(16):7833-42. doi: 10.1093/nar/16.16.7833.
Treatment of Halobacterium GRB cells with the DNA topoisomerase II inhibitor novobiocin induces the accumulation of a circular single-stranded DNA form of the plasmid pGRB-1. This form corresponds to the transcribed strand of pGRB-1. A tiny amount of this form is detectable in untreated cells. The induction of single-stranded pGRB-1 molecules by novobiocin is abolished when cells are pretreated with aphidicolin or anisomycin, which inhibit halobacterial DNA replication and protein synthesis, respectively. These results suggest that the single-stranded form of pGRB-1 is generated in the course of plasmid replication.
用DNA拓扑异构酶II抑制剂新生霉素处理嗜盐菌GRB细胞，可诱导质粒pGRB - 1的环状单链DNA形式积累。这种形式对应于pGRB - 1的转录链。在未处理的细胞中可检测到少量这种形式。当细胞分别用抑制嗜盐菌DNA复制的阿非迪霉素或抑制蛋白质合成的茴香霉素预处理时，新生霉素对单链pGRB - 1分子的诱导作用被消除。这些结果表明，pGRB - 1的单链形式是在质粒复制过程中产生的。
