Liao Chun-Hsing, Chang Chia-Lun, Huang Hsin-Hui, Lin Yi-Tsung, Li Li-Hua, Yang Tsuey-Ching
Division of Infectious Disease, Far Eastern Memorial Hospital, New Taipei City 220, Taiwan.
Department of Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.
Microorganisms. 2021 Jun 4;9(6):1216. doi: 10.3390/microorganisms9061216.
, which encodes outer membrane protein A (OmpA), is the most abundant transcript in based on transcriptome analyses. The functions of OmpA, including adhesion, biofilm formation, drug resistance, and immune response targets, have been reported in some microorganisms, but few functions are known in . This study aimed to elucidate the relationship between OmpA and swimming motility in . KJΔOmpA, an mutant, displayed compromised swimming and failure of conjugation-mediated plasmid transportation. The hierarchical organization of flagella synthesis genes in was established by referencing the model and was confirmed using mutant construction, qRT-PCR, and functional assays. Distinct from the model, , rather than and , was at the top of the flagellar regulatory cascade in . To elucidate the underlying mechanism responsible for -mediated swimming compromise, transcriptome analysis of KJ and KJΔOmpA was performed and revealed downregulation in KJΔOmpA as the key element. The involvement of in -mediated swimming compromise was verified using complementation, qRT-PCR, and function assays. Collectively, OmpA, which contributes to bacterial conjugation and swimming, is a promising target for adjuvant design in .
编码外膜蛋白A（OmpA）的基因，根据转录组分析，是[具体物种名称]中最丰富的转录本。在一些微生物中，OmpA的功能，包括黏附、生物膜形成、耐药性和免疫反应靶点，已有报道，但在[具体物种名称]中其功能知之甚少。本研究旨在阐明[具体物种名称]中OmpA与游动性之间的关系。KJΔOmpA，一种[具体物种名称]突变体，表现出受损的游动性以及接合介导的质粒转运失败。通过参考[相关物种名称]模型建立了[具体物种名称]中鞭毛合成基因的层级组织，并使用突变体构建、qRT-PCR和功能测定进行了验证。与[相关物种名称]模型不同，在[具体物种名称]中，[具体基因名称]而非[其他基因名称]和[其他基因名称]处于鞭毛调节级联的顶端。为了阐明导致[具体物种名称]介导的游动性受损的潜在机制，对KJ和KJΔOmpA进行了转录组分析，结果显示KJΔOmpA中[具体基因名称]下调是关键因素。使用[具体基因名称]互补、qRT-PCR和功能测定验证了[具体基因名称]在[具体物种名称]介导的游动性受损中的作用。总的来说，对细菌接合和游动有贡献的OmpA是[具体物种名称]中佐剂设计的一个有前景的靶点。
