Department of Biotechnology, Laboratory Science in Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.
Department of Pathology, Laboratory Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan.
Int J Mol Sci. 2022 Mar 31;23(7):3863. doi: 10.3390/ijms23073863.
is a motile, opportunistic pathogen. The flagellum, which is involved in swimming, swarming, adhesion, and biofilm formation, is considered a virulence factor for motile pathogens. Three flagellin genes, , , and , were identified from the sequenced genome. , , and formed an operon, and their encoding proteins shared 67-82% identity. Members of the operon were deleted individually or in combination to generate single mutants, double mutants, and a triple mutant. The contributions of the three flagellins to swimming, swarming, flagellum morphology, adhesion, and biofilm formation were assessed. The single mutants generally had a compromise in swimming and no significant defects in swarming, adhesion on biotic surfaces, and biofilm formation on abiotic surfaces. The double mutants displayed obvious defects in swimming and adhesion on abiotic and biotic surfaces. The flagellin-null mutant lost swimming ability and was compromised in adhesion and biofilm formation. All tested mutants demonstrated substantial but different flagellar morphologies, supporting that flagellin composition affects filament morphology. Bacterial swimming motility was significantly compromised under an oxidative stress condition, irrespective of flagellin composition. Collectively, the utilization of these three flagellins for filament assembly equips with flagella adapted to provide better ability in swimming, adhesion, and biofilm formation for its pathogenesis.
是一种运动、机会主义的病原体。鞭毛参与游泳、群集、粘附和生物膜形成，被认为是运动性病原体的毒力因子。从测序的 基因组中鉴定出了三个鞭毛基因， ， 。 ， 形成一个操纵子，它们编码的蛋白质具有 67-82%的同源性。 操纵子的成员被单独或组合删除，以产生单个突变体、双突变体和三突变体。评估了三种鞭毛蛋白对游泳、群集、鞭毛形态、粘附和生物膜形成的贡献。单个突变体通常在游泳方面存在缺陷，但在群集、在生物表面上的粘附和在非生物表面上的生物膜形成方面没有明显缺陷。双突变体在游泳和在非生物和生物表面上的粘附方面表现出明显的缺陷。鞭毛缺失突变体失去了游泳能力，并在粘附和生物膜形成方面受到影响。所有测试的突变体都表现出明显但不同的鞭毛形态，这表明鞭毛组成影响丝状体形态。无论鞭毛组成如何，细菌的游泳运动能力在氧化应激条件下都会受到严重影响。总之，这三种鞭毛蛋白的利用为 提供了适应的丝状体组装，使其在游泳、粘附和生物膜形成方面具有更好的致病能力。
