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摩洛哥眼镜蛇毒液及其组分对 3D 细胞培养肝癌的抗癌作用。

Anti-Cancer Effect of Moroccan Cobra Venom and Its Fractions against Hepatocellular Carcinoma in 3D Cell Culture.

机构信息

Laboratoire des Venins et Toxines, Département de Recherche, Institut Pasteur du Maroc, 1, Place Louis Pasteur, Casablanca 20360, Morocco.

Laboratoire Physiopathologie, Génétique Moléculaire & Biotechnologie, Faculté des Sciences Ain-Chock, Hassan II University of Casablanca, B.P 5366 Maarif, Casablanca 20000, Morocco.

出版信息

Toxins (Basel). 2021 Jun 4;13(6):402. doi: 10.3390/toxins13060402.

DOI:10.3390/toxins13060402
PMID:34199838
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8229680/
Abstract

Hepatocellular carcinoma (HCC) is the most common primary liver cancer in adults, the fifth most common malignancy worldwide and the third leading cause of cancer related death. An alternative to the surgical treatments and drugs, such as sorafenib, commonly used in medicine is necessary to overcome this public health problem. In this study, we determine the anticancer effect on HCC of Moroccan cobra venom and its fraction obtained by gel filtration chromatography against Huh7.5 cancer cell line. Cells were grown together with WI38 human fibroblast cells, LX2 human hepatic stellate cell line, and human endothelial cells (HUVEC) in MCTS (multi-cellular tumor spheroids) models. The hepatotoxicity of venom and its fractions were also evaluated using the normal hepatocytes cell line (Fa2N-4 cells). Our results showed that an anti HCC activity of Moroccan cobra venom and, more specifically, the F7 fraction of gel filtration chromatography exhibited the greatest anti-hepatocellular carcinoma effect by decreasing the size of MCTS. This effect is associated with a low toxicity against normal hepatocytes. These results strongly suggest that the F7 fraction of Moroccan cobra venom obtained by gel filtration chromatography possesses the ability to inhibit cancer cells proliferation. More research is needed to identify the specific molecule(s) responsible for the anticancer effect and investigate their mechanism of action.

摘要

肝细胞癌（HCC）是成人中最常见的原发性肝癌，是全球第五大常见恶性肿瘤，也是癌症相关死亡的第三大主要原因。为了克服这一公共卫生问题，有必要寻找替代手术治疗和索拉非尼等药物的方法，索拉非尼是医学上常用的药物之一。在这项研究中，我们确定了摩洛哥眼镜蛇毒液及其通过凝胶过滤色谱法获得的馏分对 Huh7.5 癌细胞系的抗癌作用。细胞与 WI38 人成纤维细胞、LX2 人肝星状细胞系和人内皮细胞（HUVEC）一起在 MCTS（多细胞肿瘤球体）模型中生长。还使用正常肝细胞系（Fa2N-4 细胞）评估了毒液及其馏分的肝毒性。我们的结果表明，摩洛哥眼镜蛇毒液具有抗 HCC 活性，更具体地说，凝胶过滤色谱法的 F7 馏分通过减小 MCTS 的大小显示出最强的抗肝癌作用。这种作用与对正常肝细胞的低毒性有关。这些结果强烈表明，凝胶过滤色谱法获得的摩洛哥眼镜蛇毒液的 F7 馏分具有抑制癌细胞增殖的能力。需要进一步研究以确定负责抗癌作用的特定分子（s）并研究其作用机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b69/8229680/87ca189f9434/toxins-13-00402-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b69/8229680/b303e1265aa8/toxins-13-00402-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b69/8229680/3b6ad2d0558d/toxins-13-00402-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b69/8229680/2f4a8f94593c/toxins-13-00402-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b69/8229680/87ca189f9434/toxins-13-00402-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b69/8229680/b303e1265aa8/toxins-13-00402-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b69/8229680/44cd038bd499/toxins-13-00402-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b69/8229680/494141c11006/toxins-13-00402-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b69/8229680/c318d54dd292/toxins-13-00402-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b69/8229680/3b6ad2d0558d/toxins-13-00402-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b69/8229680/2f4a8f94593c/toxins-13-00402-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b69/8229680/87ca189f9434/toxins-13-00402-g007.jpg

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