埃及眼镜蛇（Naja haje haje）毒液磷脂酶 A2：一种有前途的抗病毒药物，对猴轮状病毒和牛冠状病毒具有强大的病毒灭活活性。

Egyptian cobra (Naja haje haje) venom phospholipase A2: a promising antiviral agent with potent virucidal activity against simian rotavirus and bovine coronavirus.

机构信息

Molecular Biology Department, Biotechnology Research Institute, National Research Centre, Dokki, Cairo, 12622, Egypt.

Environmental Virology Laboratory, Water Pollution Research Department, Environment and Climate Change Research Institute, National Research Centre, Dokki, Cairo, 12622, Egypt.

出版信息

Arch Microbiol. 2022 Jul 27;204(8):526. doi: 10.1007/s00203-022-03139-7.

DOI:10.1007/s00203-022-03139-7

PMID:35895237

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9326960/

Abstract

Viral infections are linked to a variety of human diseases. Despite the achievements made in drug and vaccine development, several viruses still lack preventive vaccines and efficient antiviral compounds. Thus, developing novel antiviral agents is of great concern, particularly the natural products that are promising candidates for such discoveries. In this study, we have purified an approximately 15 kDa basic phospholipase A2 (PLA2) enzyme from the Egyptian cobra Naja haje haje venom. The purified N. haje PLA2 showed a specific activity of 22 units/mg protein against 6 units/mg protein for the whole crude venom with 3.67-fold purification. The antiviral activity of purified N. haje PLA2 has been investigated in vitro against bovine coronavirus (BCoV) and simian rotavirus (RV SA-11). Our results showed that the CC of PLA2 were 33.6 and 29 µg/ml against MDBK and MA104 cell lines, respectively. Antiviral analysis of N. haje PLA2 showed an inhibition of BCoV and RV SA-11 infections with a therapeutic index equal to 33.6 and 16, respectively. Moreover, N. haje PLA2 decreased the BCoV and RV SA-11 titers by 4.25 log TCID and 2.5 log TCID, respectively. Thus, this research suggests the potential antiviral activity of purified N. haje PLA2 against BCoV and RV SA-11 infections in vitro.

摘要

病毒感染与多种人类疾病有关。尽管在药物和疫苗开发方面取得了成就，但仍有几种病毒缺乏预防性疫苗和有效的抗病毒化合物。因此，开发新型抗病毒药物备受关注，特别是天然产物，它们是此类发现的有希望的候选物。在这项研究中，我们从埃及眼镜蛇 Naja haje haje 毒液中纯化了一种约 15 kDa 的碱性磷脂酶 A2 (PLA2) 酶。纯化的 N. haje PLA2 对 6 单位/毫克蛋白的全粗毒液具有 22 单位/毫克蛋白的比活，纯化倍数为 3.67 倍。已在体外研究了纯化的 N. haje PLA2 对牛冠状病毒 (BCoV) 和猴轮状病毒 (RV SA-11) 的抗病毒活性。我们的结果表明，PLA2 对 MDBK 和 MA104 细胞系的 CC 分别为 33.6 和 29µg/ml。N. haje PLA2 的抗病毒分析显示对 BCoV 和 RV SA-11 感染具有抑制作用，治疗指数分别为 33.6 和 16。此外，N. haje PLA2 分别使 BCoV 和 RV SA-11 的滴度降低了 4.25 log TCID 和 2.5 log TCID。因此，这项研究表明纯化的 N. haje PLA2 对 BCoV 和 RV SA-11 感染具有潜在的体外抗病毒活性。