从奶牛瘤胃液和粪便中分离的乳酸菌对苜蓿青贮饲料发酵品质、微生物群落及消化率的影响
Effects of Lactic Acid Bacteria Isolated From Rumen Fluid and Feces of Dairy Cows on Fermentation Quality, Microbial Community, and Digestibility of Alfalfa Silage.
作者信息
Guo Linna, Yao Dandan, Li Dongxia, Lin Yanli, Bureenok Smerjai, Ni Kuikui, Yang Fuyu
机构信息
College of Grassland Science and Technology, China Agricultural University, Beijing, China.
College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
出版信息
Front Microbiol. 2020 Jan 9;10:2998. doi: 10.3389/fmicb.2019.02998. eCollection 2019.
The objective of this study was to select lactic acid bacteria (LAB) isolated from the rumen fluid and feces of dairy cows, and evaluate their effects on silage quality of alfalfa after 30 or 60 days of ensiling. One hundred and four LAB strains were isolated from rumen fluid and feces of six dairy cows, of which four strains ( F1, F50, L100, and L120) and one commercial inoculant (GFG) isolated from forage were employed for further study. The silages treated with F1 had the lowest ( < 0.05) pH value and the highest ( < 0.05) lactic acid (LA) content in all treatments. Besides, higher ( < 0.05) digestibility was also observed in F1-treated silage after 60 days of ensiling. The microbial analysis showed that the abundance in the F1-treated silages increased to 60.32%, higher than other treatments (5.12-47.64%). Our research indicated that strain F1 could be an alternative silage inoculant, and dairy cows could be a source for obtaining excellent LAB for ensiling.
本研究的目的是从奶牛瘤胃液和粪便中筛选乳酸菌（LAB），并评估其在青贮30天或60天后对苜蓿青贮品质的影响。从6头奶牛的瘤胃液和粪便中分离出104株乳酸菌，其中4株（F1、F50、L100和L120）和1株从草料中分离出的商业接种剂（GFG）用于进一步研究。在所有处理中，用F1处理的青贮饲料pH值最低（<0.05），乳酸（LA）含量最高（<0.05）。此外，青贮60天后，F1处理的青贮饲料消化率也更高（<0.05）。微生物分析表明，F1处理的青贮饲料中该菌丰度增至60.32%，高于其他处理（5.12%-47.64%）。我们的研究表明，F1菌株可作为青贮接种剂的替代品，奶牛可作为获取优良青贮乳酸菌的来源。
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