Nuckowski Łukasz, Dzieszkowski Krzysztof, Rafiński Zbigniew, Studzińska Sylwia
Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, 7 Gagarin Str., PL-87-100 Toruń, Poland.
Chair of Organic Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, 7 Gagarin Str., PL-87-100 Toruń, Poland.
Materials (Basel). 2021 Jun 8;14(12):3146. doi: 10.3390/ma14123146.
Magnetic nanoparticles coated with zwitterionic poly(ionic liquid)s were applied for dispersive solid-phase extraction of oligonucleotides. The materials were synthesized by miniemulsion copolymerization of ionic liquids and divinylbenzene on magnetic nanoparticles. The functional monomers contain a positively charged imidazolium ring and one of the anionic groups: derivatives of acetate, malonate, or butyl sulfonate ions. Adsorption of unmodified DNA oligonucleotide on obtained materials was possible in ion-exchange (IE) and hydrophilic interactions (HI) mode. The adsorption in IE was possible at low pH and was almost complete. The adsorption in HI mode required the usage of appropriate addition of organic solvent but did not provide full adsorption. Studies on the desorption of the analytes included determining the impact of ammonium acetate concentration and pH and organic solvents addition on the recovery. The material containing acetic fragments as an anionic group was selected for the final procedure with the use of 10 mM ammonium acetate (pH = 9.5)/methanol (50/50, /) as an elution solution. The magnetic dispersive solid-phase extraction procedure was tested for the oligonucleotides with various modifications and lengths. Moreover, it was applied to extract DNA oligonucleotide and its synthetic metabolites from enriched human plasma without any pre-purification, with recoveries greater than 80%.
涂有两性离子聚（离子液体）的磁性纳米颗粒被用于寡核苷酸的分散固相萃取。这些材料是通过离子液体和二乙烯基苯在磁性纳米颗粒上的细乳液共聚合成的。功能单体包含带正电荷的咪唑环和一种阴离子基团：乙酸盐、丙二酸盐或丁基磺酸盐离子的衍生物。未修饰的DNA寡核苷酸在所得材料上的吸附可以通过离子交换（IE）和亲水相互作用（HI）模式实现。在IE模式下，低pH时吸附是可能的，并且几乎是完全的。HI模式下的吸附需要使用适当添加的有机溶剂，但不能实现完全吸附。对分析物解吸的研究包括确定乙酸铵浓度、pH和有机溶剂添加对回收率的影响。最终程序选择含有乙酸片段作为阴离子基团的材料，使用10 mM乙酸铵（pH = 9.5）/甲醇（50/50，v/v）作为洗脱溶液。对具有各种修饰和长度的寡核苷酸测试了磁性分散固相萃取程序。此外，它还被应用于从富集的人血浆中提取DNA寡核苷酸及其合成代谢物，无需任何预纯化，回收率大于80%。
