Aron Osakina, Wang Min, Lin Lianyu, Batool Wajjiha, Lin Birong, Shabbir Ammarah, Wang Zonghua, Tang Wei
Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
J Fungi (Basel). 2021 Jun 8;7(6):463. doi: 10.3390/jof7060463.
Glutamine is a non-essential amino acid that acts as a principal source of nitrogen and nucleic acid biosynthesis in living organisms. In , glutamine synthetase catalyzes the synthesis of glutamine. To determine the role of glutamine synthetase in the development and pathogenicity of plant fungal pathogens, we used Gln1 amino acid sequence to identify its orthologs in and named them MoGln1, MoGln2, and MoGln3. Deletion of and showed that they are not involved in the development and pathogenesis of . Conversely, Δ was reduced in vegetative growth, experienced attenuated growth on Minimal Medium (MM), and exhibited hyphal autolysis on oatmeal and straw decoction and corn media. Exogenous l-glutamine rescued the growth of Δ on MM. The Δ mutant failed to produce spores and was nonpathogenic on barley leaves, as it was unable to form an appressorium-like structure from its hyphal tips. Furthermore, deletion of altered the fungal cell wall integrity, with the Δ mutant being hypersensitive to HO. MoGln1, MoGln2, and MoGln3 are located in the cytoplasm. Taken together, our results shows that is important for vegetative growth, conidiation, appressorium formation, maintenance of cell wall integrity, oxidative stress tolerance and pathogenesis of .
谷氨酰胺是一种非必需氨基酸，在生物体中作为氮和核酸生物合成的主要来源。在[具体物种]中，谷氨酰胺合成酶催化谷氨酰胺的合成。为了确定谷氨酰胺合成酶在植物真菌病原体发育和致病性中的作用，我们使用[具体物种]的Gln1氨基酸序列来鉴定其直系同源物，并将它们命名为MoGln1、MoGln2和MoGln3。删除[具体基因]和[具体基因]表明它们不参与[具体物种]的发育和致病过程。相反，Δ[具体基因]突变体的营养生长减少，在基本培养基（MM）上生长减弱，并且在燕麦和稻草煎剂以及玉米培养基上表现出菌丝自溶。外源L - 谷氨酰胺挽救了Δ[具体基因]突变体在MM上的生长。Δ[具体基因]突变体未能产生孢子，并且在大麦叶片上无致病性，因为它无法从其菌丝尖端形成类似附着胞的结构。此外，删除[具体基因]改变了真菌细胞壁的完整性，Δ[具体基因]突变体对HO[具体物质]高度敏感。MoGln1、MoGln2和MoGln3位于细胞质中。综上所述，我们的结果表明[具体基因]对于[具体物种]的营养生长、分生孢子形成、附着胞形成、细胞壁完整性的维持、氧化应激耐受性和致病性很重要。
