Abah Felix, Kuang Yunbo, Biregeya Jules, Abubakar Yakubu Saddeeq, Ye Zuyun, Wang Zonghua
Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, College of Life Sciences & College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
The Engineering Technology Research Center of Characteristic Medicinal Plants of Fujian, College of Life Sciences, Ningde Normal University, Ningde 352100, China.
J Fungi (Basel). 2023 Apr 7;9(4):455. doi: 10.3390/jof9040455.
Mitogen-activated protein kinase (MAPK) signaling pathways are evolutionarily conserved in eukaryotes and modulate responses to both internal and external stimuli. Pmk1 and Mps MAPK pathways regulate stress tolerance, vegetative growth and cell wall integrity in and . Here, we deployed genetic and cell biology strategies to investigate the roles of the orthologs of Pmk1 and Mps1 in (herein referred to as SvPmk1 and SvMps1, respectively). Our results showed that SvPmk1 and SvMps1 are involved in hyphal development, asexual reproduction and pathogenesis in . We found that and mutants have significantly reduced vegetative growths on PDA supplemented with osmotic stress-inducing agents, compared to the wild type, with being hypersensitive to hydrogen peroxide. The two mutants failed to produce pycnidia and have reduced pathogenicity on . Unlike SvPmk1, SvMps1 was found to be indispensable for the fungal cell wall integrity. Confocal microscopic analyses revealed that and are ubiquitously expressed in the cytosol and nucleus. Taken together, we demonstrate here that SvPmk1 and SvMps1 play critical roles in the stress resistance, development and pathogenesis of .
丝裂原活化蛋白激酶（MAPK）信号通路在真核生物中具有进化保守性，并调节对内部和外部刺激的反应。Pmk1和Mps MAPK通路调节[具体物种1]中的应激耐受性、营养生长和细胞壁完整性。在这里，我们采用遗传学和细胞生物学策略来研究Pmk1和Mps1的直系同源物在[具体物种2]（在此分别称为SvPmk1和SvMps1）中的作用。我们的结果表明，SvPmk1和SvMps1参与了[具体物种2]的菌丝发育、无性繁殖和致病过程。我们发现，与野生型相比，[具体物种2]的[相关基因]突变体在添加渗透胁迫诱导剂的PDA上营养生长显著减少，其中[具体突变体1]对过氧化氢高度敏感。这两个突变体未能产生分生孢子器，并且在[具体宿主]上的致病性降低。与SvPmk1不同，SvMps1被发现对真菌细胞壁完整性不可或缺。共聚焦显微镜分析表明，[具体物种2]的[相关基因]在细胞质和细胞核中普遍表达。综上所述，我们在此证明SvPmk1和SvMps1在[具体物种2]的抗逆性、发育和致病过程中发挥关键作用。
