Faculty of Medicine, University of Maribor, 2000 Maribor, Slovenia.
Center for Physiology and Pharmacology, Medical University of Vienna, 1090 Vienna, Austria.
Cells. 2021 Jun 23;10(7):1580. doi: 10.3390/cells10071580.
Cholinergic innervation in the pancreas controls both the release of digestive enzymes to support the intestinal digestion and absorption, as well as insulin release to promote nutrient use in the cells of the body. The effects of muscarinic receptor stimulation are described in detail for endocrine beta cells and exocrine acinar cells separately. Here we describe morphological and functional criteria to separate these two cell types in situ in tissue slices and simultaneously measure their response to ACh stimulation on cytosolic Ca oscillations [Ca] in stimulatory glucose conditions. Our results show that both cell types respond to glucose directly in the concentration range compatible with the glucose transporters they express. The physiological ACh concentration increases the frequency of glucose stimulated [Ca] oscillations in both cell types and synchronizes [Ca] oscillations in acinar cells. The supraphysiological ACh concentration further increases the oscillation frequency on the level of individual beta cells, inhibits the synchronization between these cells, and abolishes oscillatory activity in acinar cells. We discuss possible mechanisms leading to the observed phenomena.
胰腺中的胆碱能神经支配控制着消化酶的释放，以支持肠道消化和吸收，以及胰岛素的释放，以促进身体细胞对营养物质的利用。我们详细描述了毒蕈碱受体刺激对内分泌β细胞和外分泌腺泡细胞的作用。在这里，我们描述了形态和功能标准，以便在组织切片中分离这两种细胞类型，并同时测量它们在刺激葡萄糖条件下对 ACh 刺激的反应，即细胞溶质 Ca 振荡[Ca]。我们的结果表明，两种细胞类型都直接对与它们表达的葡萄糖转运体相容的浓度范围内的葡萄糖作出反应。生理 ACh 浓度增加了两种细胞类型中葡萄糖刺激的[Ca]振荡的频率，并使腺泡细胞中的[Ca]振荡同步。超生理 ACh 浓度进一步增加了单个β细胞水平上的振荡频率，抑制了这些细胞之间的同步，并且使腺泡细胞中的振荡活动消失。我们讨论了导致观察到的现象的可能机制。
