State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
Biosensors (Basel). 2021 Jun 23;11(7):204. doi: 10.3390/bios11070204.
Metal-organic framework (MOF) nanozymes, as emerging members of the nanozymes, have received more and more attention due to their composition and structural characteristics. In this work, we report that mixed-valence state Ce-MOF (MVCM) has intrinsic haloperoxidase-mimicking activity. MVCM was synthesized by partial oxidation method using Ce-MOF as a precursor. In the presence of HO and Br, MVCM can catalyze oxidative bromination of chromogenic substrate phenol red (PR) to produce the blue product bromophenol blue (BrPR), showing good haloperoxidase-like activity. Because of the special chromogenic substrate, we constructed a ratiometric colorimetric-sensing platform by detecting the absorbance of the MVCM-(PR, Br) system at wavelengths of 590 and 430, for quantifying HO, where the detection limit of the HO is 3.25 μM. In addition, the haloperoxidase-mimicking mechanism of the MVCM is proposed. Moreover, through enzyme kinetics monitoring, the (HO and NHBr) of the MVCM is lower than that of cerium oxide nanomaterials, indicating that the MVCM has a stronger binding affinity for HO and NHBr than other materials. This work provides more application prospects for the development of nanozymes in the field of biosensors in the future.
金属-有机骨架（MOF）纳米酶作为纳米酶的新兴成员，由于其组成和结构特征而受到越来越多的关注。在这项工作中，我们报告混合价态铈-MOF（MVCM）具有固有过氧化物酶模拟活性。MVCM 通过使用 Ce-MOF 作为前体的部分氧化法合成。在 HO 和 Br 的存在下，MVCM 可以催化显色底物苯酚红（PR）的氧化溴化，生成蓝色产物溴苯酚蓝（BrPR），表现出良好的过氧化物酶样活性。由于特殊的显色底物，我们通过检测 MVCM-（PR、Br）系统在 590nm 和 430nm 处的吸光度构建了一种比率比色传感平台，用于定量 HO，HO 的检测限为 3.25μM。此外，还提出了 MVCM 的过氧化物酶模拟机制。此外，通过酶动力学监测，MVCM 的（HO 和 NHBr）低于氧化铈纳米材料，表明 MVCM 对 HO 和 NHBr 的结合亲和力强于其他材料。这项工作为未来纳米酶在生物传感器领域的发展提供了更多的应用前景。
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