Martinelli Ilenia, Tomassoni Daniele, Roy Proshanta, Di Cesare Mannelli Lorenzo, Amenta Francesco, Tayebati Seyed Khosrow
School of Pharmacy, University of Camerino, 62032 Camerino, Italy.
School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy.
Antioxidants (Basel). 2021 Jun 23;10(7):1006. doi: 10.3390/antiox10071006.
Renal and cardiac impairments are frequent events in the presence of hypertension. Organ damage is mainly linked to oxidative stress due to high blood pressure and may be reduced by antioxidant supplementation. Alpha-lipoic acid (ALA) is one of most effective antioxidants. It is widely used as a nutritional supplement in a racemic mixture (+/-), even though the (+)-enantiomer is biologically active. This study was designed to investigate the effect of treatment with (+/-)-ALA and its enantiomers on renal and heart parenchyma in spontaneously hypertensive rats (SHR), using immunochemical and immunohistochemical techniques. The results confirmed that the oxidative mechanisms of organ alterations, due to hypertension, and characterized by glomerular and tubular lesions, left ventricular hypertrophy, and fibrosis but not by apoptosis were accompanied by proteins' and nucleic acids' oxidation. We found greater effectiveness of (+)-ALA compared to (+/-)-ALA in reducing oxidative stress, cardiac and renal damages in SHR. To conclude, these data propose (+)-ALA as one of the more appropriate antioxidant molecules to prevent renal and cardiac alterations associated with hypertension.
在高血压患者中，肾脏和心脏损害是常见事件。器官损伤主要与高血压引起的氧化应激有关，补充抗氧化剂可能会减轻这种损伤。α-硫辛酸（ALA）是最有效的抗氧化剂之一。尽管（+）-对映体具有生物活性，但它仍以消旋混合物（+/-）的形式被广泛用作营养补充剂。本研究旨在利用免疫化学和免疫组织化学技术，研究（+/-）-ALA及其对映体治疗对自发性高血压大鼠（SHR）肾脏和心脏实质的影响。结果证实，高血压引起的器官改变的氧化机制，其特征为肾小球和肾小管病变、左心室肥大和纤维化而非细胞凋亡，伴有蛋白质和核酸的氧化。我们发现，与（+/-）-ALA相比，（+）-ALA在减轻SHR的氧化应激、心脏和肾脏损伤方面更有效。总之，这些数据表明（+）-ALA是预防与高血压相关的肾脏和心脏改变的更合适的抗氧化分子之一。
