Institute of Microbiology, Infectious Diseases and Immunology, Gastrointestinal Microbiology Research Group, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, D-12203 Berlin, Germany.
Int J Mol Sci. 2021 Jun 22;22(13):6683. doi: 10.3390/ijms22136683.
Prevalences of infections are progressively rising globally. Given that probiotic feed additives, such as the commercial product Aviguard, have been shown to be effective in reducing enteropathogens, such as , in vertebrates, including livestock, we assessed potential anti-pathogenic and immune-modulatory properties of Aviguard during acute -induced murine enterocolitis. Therefore, microbiota-depleted IL-10 mice were infected with strain 81-176 by gavage and orally treated with Aviguard or placebo from day 2 to 4 post-infection. The applied probiotic bacteria could be rescued from the intestinal tract of treated mice, but with lower obligate anaerobic bacterial counts in -infected as compared to non-infected mice. Whereas comparable gastrointestinal pathogen loads could be detected in both groups until day 6 post-infection, Aviguard treatment resulted in improved clinical outcome and attenuated apoptotic cell responses in infected large intestines during acute campylobacteriosis. Furthermore, less distinct pro-inflammatory immune responses could be observed not only in the intestinal tract, but also in extra-intestinal compartments on day 6 post-infection. In conclusion, we show here for the first time that Aviguard exerts potent disease-alleviating effects in acute -induced murine enterocolitis and might be a promising probiotic treatment option for severe campylobacteriosis in humans.
感染的流行率在全球范围内呈逐渐上升趋势。鉴于益生菌饲料添加剂，如商业产品 Aviguard，已被证明可有效减少脊椎动物（包括牲畜）中的肠道病原体，如 ，我们评估了 Aviguard 在急性 诱导的小鼠肠炎中的潜在抗病原体和免疫调节特性。因此，用抗生素处理耗尽肠道微生物群的 IL-10 小鼠，通过灌胃感染 菌株 81-176，并在感染后第 2 至 4 天用 Aviguard 或安慰剂进行口服治疗。可从治疗后的小鼠肠道中回收应用的益生菌细菌，但与未感染的小鼠相比，感染的小鼠肠道中必需的厌氧菌计数较低。虽然在两组中都可检测到相似的胃肠道病原体负荷，直至感染后第 6 天，但 Aviguard 治疗可改善临床结果，并减轻急性弯曲杆菌病感染大肠中的细胞凋亡反应。此外，不仅在肠道中，而且在感染后第 6 天的肠外部位也可观察到不那么明显的促炎免疫反应。总之，我们首次证明，Aviguard 在急性 诱导的小鼠肠炎中具有显著的疾病缓解作用，并且可能是人类严重弯曲杆菌病的一种有前途的益生菌治疗选择。
