An Jiae, Kim Pyoung Beom, Park Hyeong Bin, Kim Seongjun, Park Hwan Joon, Lee Chang Woo, Lee Byoung-Doo, Kim Nam Young, Hwang Jung Eun
Division of Restoration Research, Research Center for Endangered Species, National Institute of Ecology, Yeongyang 36531, Korea.
Department of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Korea.
Plants (Basel). 2021 Jun 11;10(6):1193. doi: 10.3390/plants10061193.
is becoming endangered, and even extinct, due to habitat destruction and illegal collection, and the development of an optimized artificial propagation system is necessary for its conservation and reintroduction. Thus, the effects of plant growth medium strength (Murashige and Skoog (MS) and Hyponex media) and the addition of activated charcoal (AC) and organic supplements on seedling growth of were investigated through in vitro seed culture. The results showed that seedling growth was higher in half-strength (1/2) media than in full-strength media. After the addition of AC, the highest leaf area (2.14 cm) was recorded in the seedlings grown in 1/2 Hyponex medium, and after the addition of organic supplements, root development increased regardless of the media type. Among the sixteen suitable media tested at later seedling growth stages, 1/2 MS medium with the addition of 0.6 g·L AC, 30 g·L banana homogenate and 10 g·L apple homogenate was generally effective in fresh weight (6.13 g) and root length (9.59 cm). We demonstrated which organic supplements are preferred for in vitro growth of seedlings developed from protocorms by asymbiotic seed culture, which can be used for mass production and conservation of this rare epiphytic orchid.
由于栖息地破坏和非法采集，它正濒临灭绝甚至已经灭绝，因此，开发优化的人工繁殖系统对其保护和重新引入至关重要。因此，通过离体种子培养研究了植物生长培养基强度（Murashige和Skoog（MS）培养基以及花宝培养基）以及添加活性炭（AC）和有机添加物对[植物名称]幼苗生长的影响。结果表明，半强度（1/2）培养基中的幼苗生长高于全强度培养基。添加AC后，在1/2花宝培养基中生长的幼苗记录到最高叶面积（2.14平方厘米），添加有机添加物后，无论培养基类型如何，根系发育均有所增加。在后期幼苗生长阶段测试的16种合适培养基中，添加0.6克·升AC、30克·升香蕉匀浆和1Og·升苹果匀浆的1/2 MS培养基在鲜重（6.13克）和根长（9.59厘米）方面通常效果良好。我们证明了通过非共生种子培养，哪种有机添加物最适合从[植物名称]原球茎发育而来的幼苗的离体生长，这可用于这种珍稀附生兰花的大规模生产和保护。
