Pedroso Adriana A, Lee Margie D, Maurer John J
Department of Population Health, University of Georgia, Athens, GA, United States.
Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States.
Front Microbiol. 2021 Jun 15;12:694215. doi: 10.3389/fmicb.2021.694215. eCollection 2021.
The transfer of the intestinal microbiota from adult to juvenile animals reduces prevalence and abundance. The mechanism behind this exclusion is unknown, however, certain member species may exclude or promote pathogen colonization and abundance in chickens correlates with intestinal community composition. In this study, newly hatched chicks were colonized with Typhimurium and 16S rRNA libraries were generated from the cecal bacterial community at 21, 28, 35, and 42 days of age. was quantified by real-time PCR. Operational taxonomic units (OTUs) were assigned, and taxonomic assignments were made, using the Ribosomal Database Project. Bacterial diversity was inversely proportional to the abundance in the chicken cecum ( < 0.01). In addition, cecal communities with no detectable (exclusive community) displayed an increase in the abundance of OTUs related to specific clostridial families (, , and ), genera ( and ) and member species (, , and ). For cecal communities with high abundance (permissive community), there was a positive correlation with the presence of unclassified , clostridial genera and clostridial species , , and . These findings strongly support the link between the intestinal bacterial species diversity and the presence of specific member species with abundance in the chicken ceca. Exclusive bacterial species could prove effective as direct-fed microbials for reducing in poultry while permissive species could be used to predict which birds will be super-shedders.
将成年动物的肠道微生物群转移到幼年动物体内会降低其流行率和丰度。然而，这种排斥背后的机制尚不清楚，某些成员物种可能会排斥或促进病原体的定殖，并且鸡体内的[病原体名称未明确]丰度与肠道群落组成相关。在本研究中，刚孵化出的雏鸡用鼠伤寒沙门氏菌进行定殖，并在21、28、35和42日龄时从盲肠细菌群落中生成16S rRNA文库。通过实时PCR对[病原体名称未明确]进行定量。使用核糖体数据库项目指定操作分类单元（OTU）并进行分类学分配。细菌多样性与鸡盲肠中[病原体名称未明确]的丰度成反比（P<0.01）。此外，未检测到[病原体名称未明确]的盲肠群落（排他性群落）显示，与特定梭菌科（[科名未明确]、[科名未明确]和[科名未明确]）、属（[属名未明确]和[属名未明确]）以及成员物种（[物种名未明确]、[物种名未明确]和[物种名未明确]）相关的OTU丰度增加。对于[病原体名称未明确]丰度高的盲肠群落（允许性群落），与未分类的[细菌名称未明确]、梭菌属[属名未明确]以及梭菌物种[物种名未明确]、[物种名未明确]和[物种名未明确]的存在呈正相关。这些发现有力地支持了鸡盲肠中肠道细菌物种多样性与具有[病原体名称未明确]丰度的特定成员物种存在之间的联系。排他性细菌物种可能被证明作为直接投喂微生物在降低家禽体内[病原体名称未明确]方面有效，而允许性物种可用于预测哪些鸟类将成为超级排泄者。
