Department of Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
College of Public Health Sciences, Chulalongkorn University (CPHS), Bangkok, Thailand.
Sci Rep. 2021 Jul 2;11(1):13753. doi: 10.1038/s41598-021-93263-z.
Lactic acid bacteria (LAB) are used as a probiotic alternative to antibiotics in livestock production. Microencapsulation technology is widely used for probiotic preservation. A variety of microencapsulation protocols have been proposed and compared based on chemicals and mechanical procedures. This study aimed to develop a double-encapsulated coating from alginate (1.5%) and chitosan (0.5%) by extrusion, emulsion, and spray drying methods using the LAB strains Lactobacillus plantarum strains 31F, 25F, 22F, Pediococcus pentosaceus 77F, and P. acidilactici 72N, and to monitor the basic probiotic properties of the encapsulated prototypes. The final products from each microencapsulation protocol were analysed for their appearance, probiotic properties and viable cell count. Using the spray drying method, particles smaller than 15 μm in diameter with a regular spherical shape were obtained, whereas the other methods produced larger (1.4-52 mm) and irregularly shaped microcapsules. After storage for 6 months at room temperature, the LAB viability of the spray-dried particles was the highest among the three methods. In all the LAB strains examined, the encapsulated LAB retained their probiotic properties in relation to acid-bile tolerance and antibacterial activity. This study highlights the efficacy of double-coating microencapsulation for preserving LAB properties and survival rate, and demonstrates its potential for probiotic application in livestock farms.
乳酸菌（LAB）被用作替代抗生素的益生菌，用于畜牧业生产。微胶囊技术广泛用于益生菌的保存。已经提出并比较了各种基于化学物质和机械程序的微胶囊化方案。本研究旨在通过挤压、乳化和喷雾干燥方法，使用 LAB 菌株植物乳杆菌 31F、25F、22F、戊糖片球菌 77F 和嗜酸乳杆菌 72N，用海藻酸钠（1.5%）和壳聚糖（0.5%）开发双层包衣，并监测封装原型的基本益生菌特性。对每种微胶囊化方案的最终产物进行外观、益生菌特性和活菌计数分析。使用喷雾干燥方法，获得了直径小于 15μm、具有规则球形的颗粒，而其他方法则产生了较大（1.4-52mm）和不规则形状的微胶囊。在室温下储存 6 个月后，喷雾干燥颗粒中 LAB 的存活率在三种方法中最高。在所检查的所有 LAB 菌株中，包封的 LAB 在耐酸碱性和抗菌活性方面保留了其益生菌特性。本研究强调了双层包衣微胶囊化在保存 LAB 特性和存活率方面的功效，并展示了其在养殖场中应用于益生菌的潜力。
