Department of Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
College of Public Health Sciences, Chulalongkorn University (CPHS), Bangkok, Thailand.
Sci Rep. 2021 Jun 8;11(1):12028. doi: 10.1038/s41598-021-91427-5.
The lactic acid bacteria (LAB) Lactobacillus plantarum (strains 22F and 25F) and Pediococcus acidilactici (strain 72N) have appeared promising as replacements for antibiotics in in vitro studies. Microencapsulation, especially by the spray-drying method, has been used to preserve their numbers and characteristics during storage and digestion. This study compared the efficacy of these strains and their microencapsulated form with antibiotic usage on growth performance, faecal microbial counts, and intestinal morphology in nursing-finishing pigs. A total of 240 healthy neonatal pigs were treated on days 0, 3, 6, 9, and 12 after cross-fostering. Sterile peptone water was delivered orally to the control and antibiotic groups. Spray-dried Lactobacillus plantarum strain 22F stored for 6-months was administered to piglets in the spraydry group. Three ml of each the three fresh strains (10 CFU/mL) were orally administered to piglets in each group. All pigs received the basal diets, but these were supplemented with routine antibiotic for the antibiotic group. Pigs in all the probiotic supplemented groups exhibited a better average daily gain and feed conversion ratio than those of the controls in the nursery and grower phases. Probiotic supplementation increased viable lactobacilli and decreased enterobacterial counts. Antibiotic additives reduced both enterobacterial and lactobacilli counts. Villous height and villous height:crypt depth ratio were greater in probiotic and antibiotic supplemented pigs comparing to the controls, especially in the jejunum. The results demonstrated the feasibility of using these strains as a substitute for antibiotics and the practicality of the microencapsulation protocol for use in swine farms.
乳酸菌（LAB）植物乳杆菌（菌株 22F 和 25F）和戊糖片球菌（菌株 72N）在体外研究中作为抗生素替代品表现出良好的效果。微胶囊化，特别是喷雾干燥法，已被用于在储存和消化过程中保持其数量和特性。本研究比较了这些菌株及其与抗生素使用相比在生长性能、粪便微生物计数和哺乳仔猪肠道形态方面的微胶囊化形式的效果。共处理 240 头健康新生仔猪，在寄养后第 0、3、6、9 和 12 天进行处理。无菌蛋白胨水被口服给予对照组和抗生素组。喷雾干燥保存 6 个月的植物乳杆菌 22F 菌株被施用于喷雾干燥组的仔猪。每组仔猪口服给予三种新鲜菌株各 3ml（10 CFU/mL）。所有仔猪均接受基础日粮，但抗生素组补充常规抗生素。在保育和生长阶段，所有添加益生菌的猪的平均日增重和饲料转化率均优于对照组。益生菌补充剂增加了乳酸菌的活菌数，降低了肠杆菌的数量。抗生素添加剂减少了肠杆菌和乳酸菌的数量。与对照组相比，益生菌和抗生素添加猪的绒毛高度和绒毛高度：隐窝深度比值更大，尤其是在空肠。结果表明，这些菌株作为抗生素替代品是可行的，并且微胶囊化方案在养猪场中的实用性。
