Roelfes Gerard
Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, 9747, AG, Groningen, the Netherlands.
J Inorg Biochem. 2021 Sep;222:111523. doi: 10.1016/j.jinorgbio.2021.111523. Epub 2021 Jun 23.
Heme enzymes are some of the most versatile catalysts in nature. In recent years it has been found that they can also catalyze reactions for which there are no equivalents in nature. This development has been driven by the abiological catalytic reactivity reported for bio-inspired and biomimetic iron porphyrin complexes. This review focuss es on heme enzymes for catalysis of cyclopropanation reactions. The two most important approaches used to create enzymes for cyclopropanation are repurposing of heme enzymes and the various strategies used to improve these enzymes such as mutagenesis and heme replacement, and artificial heme enzymes. These strategies are introduced and compared. Moreover, lessons learned with regard to mechanism and design principles are discussed.
血红素酶是自然界中一些最为通用的催化剂。近年来，人们发现它们还能催化自然界中不存在对应反应的化学反应。这一进展是由生物启发和仿生铁卟啉配合物所报道的非生物催化反应活性推动的。本综述聚焦于催化环丙烷化反应的血红素酶。用于创建环丙烷化酶的两种最重要方法是血红素酶的重新利用以及用于改进这些酶的各种策略，如诱变和血红素置换，还有人工血红素酶。本文将介绍并比较这些策略。此外，还将讨论在反应机理和设计原则方面所获得的经验教训。
