Rahman Afiqah Zafirah Abdul, Adzahan Noraniza Mohd, Zakaria Zunita, Mayaki Abubakar Musa
Department of Farm and Exotic Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Vet World. 2021 May;14(5):1128-1132. doi: 10.14202/vetworld.2021.1128-1132. Epub 2021 May 10.
Horse wounds can be easily infected with bacteria depending on the nature of its cause such as laceration, abrasion, or puncture as well as the nature of its environment. Various treatments are available in managing open wounds, including the usage of topical antibiotics and antiseptics. However, antibiotic resistance has been a major concern attributed with chronic wound infection. The aim of this study was to test the efficacy of ionized water at different pH against the growth of common bacteria from horse wounds.
Ten swab samples from equine infected wounds were collected and bacteria isolation and identification were performed. The antibacterial effect of the ionized water of pH 2.5, 4.5, 7.0, and 11.5 was tested on , , , , , and . The time-kill profiles of the ionized waters were determined at time 0, 2, 4, 6, and 8 h.
Ionized water of pH 2.5 and 4.5 showed antibacterial activity against , , and with significant (p>0.05) reduction in colony-forming unit/mL within 2-8 h. The degree of bactericidal effect of the acidic ionized water differs between the species with more susceptible. However, there was no antibacterial effect at pH 2.5, 4.5, 7.0, and 11.5 on the Gram-negative bacteria tested.
Ionized water of pH 2.5 and 4.5 is effective in minimizing the growth of Gram-positive bacteria; thus it could be of clinical importance as an antiseptic for surface wound lavage in horses.
根据马伤口的致伤原因（如撕裂伤、擦伤或刺伤）以及环境性质，马伤口很容易被细菌感染。在处理开放性伤口时，有多种治疗方法可供选择，包括使用局部抗生素和防腐剂。然而，抗生素耐药性一直是慢性伤口感染的一个主要问题。本研究的目的是测试不同pH值的离子水对马伤口常见细菌生长的有效性。
收集10份马感染伤口的拭子样本，进行细菌分离和鉴定。测试了pH值为2.5、4.5、7.0和11.5的离子水对[具体细菌名称未给出]、[具体细菌名称未给出]、[具体细菌名称未给出]、[具体细菌名称未给出]、[具体细菌名称未给出]和[具体细菌名称未给出]的抗菌效果。在0、2、4、6和8小时测定离子水的杀菌时间曲线。
pH值为2.5和4.5的离子水对[具体细菌名称未给出]、[具体细菌名称未给出]和[具体细菌名称未给出]具有抗菌活性，在2至8小时内菌落形成单位/毫升显著降低（p>0.05）。酸性离子水的杀菌效果在不同菌种之间有所不同，[具体细菌名称未给出]更易受影响。然而，pH值为2.5、4.5、7.0和11.5的离子水对所测试的革兰氏阴性菌没有抗菌作用。
pH值为2.5和4.5的离子水可有效减少革兰氏阳性菌的生长；因此，作为马体表伤口冲洗的防腐剂，它可能具有临床重要性。
