Güneş Eda, Aydin Hasan, Nizamlioğlu Hatice Ferhan
Department of Gastronomy and Culinary Arts, Faculty of Tourism, Necmettin Erbakan University, Konya, Turkey.
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Adiyaman University, Adiyaman, Turkey.
Toxicol Rep. 2021 Jun 16;8:1300-1304. doi: 10.1016/j.toxrep.2021.06.014. eCollection 2021.
Sulfonamide-based compounds in the development of drugs used in cancer treatment have been started to be investigated recently. In the current work, it was determined the protective effect of Acetazolamide (AAZ) and SLC-0111 on carbon tetrachloride-induced toxicity in the fruit fly (). AAZ and SLC-0111 were used as a nonselective and selective inhibitor of carbonic anhydrase isozymes, respectively, to compare the selectivity effect of drugs on toxicity. The experimental toxicity was created by carbon tetrachloride (CCl) that causes tissue damage to the first stage larvae of fruit fly and used as a model organism. The effect of AAZ and SLC-0111 on toxicity of insect survival, sex ratio, longevity and some biochemical parameters such as Malondialdehyde-MDA content, Superoxide dismutase-SOD and Glutathione--transferase-GST activity were tested. According to the data obtained, feeding of insects with AAZ and SLC-0111 (2.5 and 10 mM, respectively) affected their survival and development positively against the toxicity induced by CCl. Compared to the control group, GST and SOD activity was higher in pups and adults (SLC-0111 < AAZ). Because of this study, SLC-0111 is thought to be useful in protecting against the harmful effects of reactive oxygen species.
近年来，人们开始研究用于癌症治疗的基于磺胺类的化合物。在当前的工作中，确定了乙酰唑胺（AAZ）和SLC-0111对四氯化碳诱导的果蝇毒性的保护作用。分别使用AAZ和SLC-0111作为碳酸酐酶同工酶的非选择性和选择性抑制剂，以比较药物对毒性的选择性作用。通过四氯化碳（CCl）对果蝇第一阶段幼虫造成组织损伤来产生实验性毒性，并将果蝇用作模式生物。测试了AAZ和SLC-0111对昆虫存活毒性、性别比例、寿命以及一些生化参数（如丙二醛-MDA含量、超氧化物歧化酶-SOD和谷胱甘肽-S-转移酶-GST活性）的影响。根据获得的数据，用AAZ和SLC-0111（分别为2.5和10 mM）喂养昆虫对CCl诱导的毒性对其存活和发育有积极影响。与对照组相比，幼崽和成虫中的GST和SOD活性更高（SLC-0111 < AAZ）。由于这项研究，SLC-0111被认为有助于抵御活性氧物种的有害影响。
