Goff W, Barbet A, Stiller D, Palmer G, Knowles D, Kocan K, Gorham J, McGuire T
Animal Diseases Research Unit, Washington State University, Pullman 99164.
Proc Natl Acad Sci U S A. 1988 Feb;85(3):919-23. doi: 10.1073/pnas.85.3.919.
Anaplasmosis is the most widely distributed of several important tick-borne diseases that constrain cattle production throughout much of the world. Evaluation of the effectiveness of disease control strategies that integrate vaccination with tick control requires the ability to monitor tick and cattle infection rates. To detect Anaplasma marginale in ticks and bovine erythrocytes, a 2-kilobase DNA fragment from a cloned A. marginale gene coding for a surface protein having a Mr of 105,000 was prepared and evaluated as a probe. The probe was species specific and detected A. marginale DNA derived from infected bovine erythrocytes and adult Dermacentor ticks infected either as nymphs or adults. Tick infection was confirmed by microscopy and test feeding on a susceptible calf. The sensitivity of the probe is suitable for detecting infected ticks in experimental and field epizootiology studies.
无形体病是几种重要的蜱传疾病中分布最广的一种，这些疾病在世界大部分地区制约着养牛业的发展。评估将疫苗接种与蜱虫控制相结合的疾病控制策略的有效性，需要具备监测蜱虫和牛感染率的能力。为了检测蜱虫和牛红细胞中的边缘无形体，制备了一个来自克隆的边缘无形体基因的2千碱基DNA片段，该基因编码一种分子量为105,000的表面蛋白，并将其作为探针进行评估。该探针具有种属特异性，可检测来自感染牛红细胞以及若虫或成虫阶段感染的成年微小牛蜱的边缘无形体DNA。通过显微镜检查和在易感小牛身上进行试验性喂食来确认蜱虫感染情况。该探针的灵敏度适用于在实验性和现场动物流行病学研究中检测受感染的蜱虫。
