评价 2 种商业抗 SARS-CoV-2 抗体检测试剂盒在伴有 COVID-19 的免疫功能正常和免疫功能低下住院患者中的应用。
Evaluation of 2 commercial anti-SARS-CoV-2 antibody assays in an immunocompetent and immunocompromised inpatient population with COVID-19.
机构信息
Department of Virology, Barts Health NHS Trust, London, UK.
Department of Virology, Barts Health NHS Trust, London, UK.
出版信息
Diagn Microbiol Infect Dis. 2021 Oct;101(2):115449. doi: 10.1016/j.diagmicrobio.2021.115449. Epub 2021 Jun 5.
INTRODUCTION
During the COVID-19 pandemic, widespread introduction of SARS-CoV-2 antibody testing was introduced without a full understanding of the assays performance or the antibody kinetics following infection with SARS-CoV-2.
METHODS
We performed an evaluation of 2 anti-SARS-CoV-2 antibody assays with a more detailed look into the effect of immune status on antibody sensitivity.
RESULTS
Both assays demonstrated 100% specificity. The overall sensitivity of the Roche was 92.1% at ≥14 days and 94.8% at ≥21 days, and the overall sensitivity of the Abbott was 94.4% at ≥14 days and 98.2% at ≥21 days. 7/41 (17%) of patients included in this cohort were immunocompromised. Seroconversion was seen less commonly in the immunocompromised (4/7 [57.1%] seroconverted) and after excluding these patients 100% sensitivity was seen in both assays at ≥21 days.
DISCUSSION
Performance of both assays in the immunocompetent appeared excellent after 21 days postsymptom onset. Both assays are highly specific.
简介
在 COVID-19 大流行期间，尽管我们对 SARS-CoV-2 抗体检测的性能以及感染 SARS-CoV-2 后的抗体动力学没有充分的了解，但还是广泛引入了 SARS-CoV-2 抗体检测。
方法
我们对 2 种抗 SARS-CoV-2 抗体检测方法进行了评估，更详细地研究了免疫状态对抗体敏感性的影响。
结果
两种检测方法均显示出 100%的特异性。罗氏检测的总体敏感性在≥14 天和≥21 天分别为 92.1%和 94.8%，雅培检测的总体敏感性在≥14 天和≥21 天分别为 94.4%和 98.2%。本队列中 7/41（17%）名患者免疫功能低下。在免疫功能低下的患者中，血清转化较少见（7/7 [57.1%] 血清转化），排除这些患者后，两种检测方法在≥21 天的敏感性均为 100%。
讨论
在症状出现后 21 天，两种检测方法在免疫功能正常的患者中的表现均非常出色。两种检测方法均具有很高的特异性。
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