Dimitrov O A, Kabaivanova S D, Nikolov I T, Ivanova V L, Moskova V V
Probl Endokrinol (Mosk). 1987 Sep-Oct;33(5):56-60.
The effect of estradiol on DNA synthesis in hepatocytes was investigated for a study of the functional importance and mechanism of action of sex steroids in the liver. After administration of 17, beta-estradiol dipropionate at a dose of 20 micrograms per 100 g of body mass to ovariectomized albino female rats for 11 days the kinetics of incorporation of 3H-thymidine triphosphate in liver cell nuclei and chromatin was determined. A stimulating effect of the hormone on the tracer incorporation in the nuclei and chromatin was established. A similar tendency was noted in immature female rats. The tracer incorporation depends on the cytosol presence in the system. The rate of incorporation has been shown to increase under the influence of the liver cytosol of intact rats. In can be assumed that estradiol is involved in the regulation of liver DNA synthesis raising matrix activity and modulating cytosol factor activity.
为研究性类固醇在肝脏中的功能重要性及作用机制，对雌二醇对肝细胞DNA合成的影响进行了研究。以每100克体重20微克的剂量给去卵巢白化雌性大鼠注射17β - 二丙酸雌二醇，持续11天，然后测定肝细胞细胞核和染色质中3H - 三磷酸胸腺嘧啶核苷掺入的动力学。确定了该激素对示踪剂掺入细胞核和染色质有刺激作用。在未成熟雌性大鼠中也观察到类似趋势。示踪剂掺入取决于系统中胞质溶胶的存在。已表明在完整大鼠肝脏胞质溶胶的影响下掺入率会增加。可以假定雌二醇参与肝脏DNA合成的调节，提高模板活性并调节胞质溶胶因子活性。
