Toschi V, Renoldi P, Motta A, Cimminiello C, Arpaia G, Fiorini G F
Department of Haematology, San Carlo General Hospital, Milan, Italy.
Rheumatol Int. 1987;7(5):213-6. doi: 10.1007/BF00541379.
Plasma fibronectin (FN) was measured in 17 patients with essential mixed cryoglobulinaemia (EMC) and in 17 normal subjects by single radial immunodiffusion (RID) and enzyme-linked immunosorbent assay (ELISA). In 9 patients the presence of FN in the cryoprecipitates was also assessed by immunoblotting. In the EMC group, plasma FN levels were significantly lower than in control subjects, using both methods, and FN was constantly demonstrated in EMC cryoprecipitates. Capillaroscopic observation of the capillary bed in skin and bulbar conjunctiva, performed in all cases, showed severe alterations of microcirculation in EMC patients. A negative correlation between plasma FN and capillaroscopic abnormalities of skin capillaries was observed. These data support the hypothesis that plasma fibronectin plays a role in the pathogenesis of systemic vasculitis in EMC.
采用单向免疫扩散法（RID）和酶联免疫吸附测定法（ELISA）对17例原发性混合性冷球蛋白血症（EMC）患者和17名正常受试者的血浆纤维连接蛋白（FN）进行了检测。对9例患者的冷沉淀物中FN的存在情况也通过免疫印迹法进行了评估。在EMC组中，两种方法均显示血浆FN水平显著低于对照组，并且在EMC冷沉淀物中持续检测到FN。对所有病例进行的皮肤和球结膜毛细血管床的毛细血管镜观察显示，EMC患者存在严重的微循环改变。观察到血浆FN与皮肤毛细血管的毛细血管镜异常之间呈负相关。这些数据支持了血浆纤维连接蛋白在EMC系统性血管炎发病机制中起作用这一假说。
