State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.
College of Chemistry and Environmental Sciences, Laboratory of Xinjiang Native Medicinal and Edible Plant Resources Chemistry, Kashgar University, Kashgar 844000, People's Republic of China.
Food Chem. 2021 Dec 1;364:130310. doi: 10.1016/j.foodchem.2021.130310. Epub 2021 Jun 6.
Stevia rebaudiana, a sweetener with medicinal functions, has attracted extensive attention due to its application in food and pharmaceutical fields. However, a few studies were performed to explore polysaccharides in this plant. Herein, SRP70-1 was derived from S. rebaudiana. Structural analysis (monosaccharide composition analysis, high-performance liquid chromatography-multi-angle light scattering detection, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy) revealed that SRP70-1 was composed of mannose, glucose, galactose, and arabinose at the molar ratio of 1.35:1.00:3.23:3.47, with an absolute molecular weight of 7698 Da. SRP70-1 was found to contain → 5)-α-l-Araf-(1→, →2,3,5)-α-l-Araf-(1→, →4)-β-l-Arap-(1→, →4)-β-d-Galp-(1→, →6)-β-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-β-d-Manp-(1→, and terminal α-l-Araf, β-d-Galp, and β-d-Glcp residues. Cell experiments showed that SRP70-1 could significantly promote phagocytosis and increase the release of nitric oxide and cytokines including IL-1β, IL-6, and TNF-α. Further zebrafish experiments confirmed the immunological enhancement effects of SRP70-1. This study revealed that SRP70-1 may be useful for the development of functional foods.
甜菊糖 Rebaudioside A，一种具有药用功能的甜味剂，由于其在食品和制药领域的应用而受到广泛关注。然而，目前对该植物中的多糖研究较少。本研究从甜叶菊中提取得到 SRP70-1。结构分析（单糖组成分析、高效液相色谱-多角度激光散射检测、气相色谱-质谱联用和核磁共振波谱）表明，SRP70-1 由摩尔比为 1.35:1.00:3.23:3.47 的甘露糖、葡萄糖、半乳糖和阿拉伯糖组成，其重均分子量为 7698 Da。SRP70-1 含有→5)-α-l-阿拉伯呋喃糖基-(1→, →2,3,5)-α-l-阿拉伯呋喃糖基-(1→, →4)-β-l-阿拉伯吡喃糖基-(1→, →4)-β-d-吡喃半乳糖基-(1→, →6)-β-d-吡喃半乳糖基-(1→, →4)-β-d-吡喃甘露糖基-(1→, →6)-β-d-吡喃甘露糖基-(1→和末端α-l-阿拉伯呋喃糖基、β-d-吡喃半乳糖基和β-d-吡喃葡萄糖基残基。细胞实验表明，SRP70-1 能显著促进吞噬作用，增加一氧化氮和细胞因子（包括 IL-1β、IL-6 和 TNF-α）的释放。进一步的斑马鱼实验证实了 SRP70-1 的免疫增强作用。本研究表明，SRP70-1 可能有助于功能性食品的开发。
