黄韧伞真菌多糖的结构、抗肿瘤活性及其潜在抗肿瘤机制。
Structure, anti-tumor activity, and potential anti-tumor mechanism of a fungus polysaccharide from Fomes officinalis.
机构信息
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China; Tianjin International Joint Academy of Biomedicine, Tianjin 300457, People's Republic of China.
出版信息
Carbohydr Polym. 2022 Nov 1;295:119794. doi: 10.1016/j.carbpol.2022.119794. Epub 2022 Jun 30.
In our ongoing process of discovering bioactive macromolecules, a homogeneous polysaccharide (FOP80-1) was first purified from Fomes officinalis. FOP80-1 with molecular weight of 4560 Da was mainly composed of →3)-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-α-d-Glcp-(1→, →3,6)-d-Glcp-(1→, and t--d-Glcp. Besides the structure features, the anti-tumor activity and potential mechanism of FOP80-1 were also investigated. The cellular and zebrafish experiments revealed that FOP80-1 inhibited tumor proliferation, invasion, and metastasis by increasing ROS, arresting cell cycle, inducing apoptosis, and suppressing angiogenesis. Corresponding to the inhibition of angiogenesis, the surface plasmon resonance (SPR) experiments revealed that FOP80-1 had good affinity with VEGF, a crucial protein to regulate angiogenesis. Molecular docking indicated that FOP80-1 could interact with the protein VEGF.
在我们不断探索生物活性大分子的过程中，首次从红栓菌（Fomes officinalis）中纯化出一种均一的多糖（FOP80-1）。FOP80-1 的分子量为 4560 Da，主要由 →3）-d-Galp-(1→、→4)-β-d-Manp-(1→、→6)-α-d-Glcp-(1→、→3,6)-d-Glcp-(1→和 t--d-Glcp 组成。除了结构特征外，还研究了 FOP80-1 的抗肿瘤活性及其潜在机制。细胞和斑马鱼实验表明，FOP80-1 通过增加 ROS、细胞周期阻滞、诱导细胞凋亡和抑制血管生成来抑制肿瘤增殖、侵袭和转移。与抑制血管生成相对应，表面等离子体共振（SPR）实验表明，FOP80-1 与 VEGF 具有良好的亲和力，VEGF 是调节血管生成的关键蛋白。分子对接表明，FOP80-1 可以与 VEGF 蛋白相互作用。
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