Huang Fuda, Li Hua, Qin Zebang, Wang Anmin, Zhang Ya, Guo Junyu, Wei Mingwei, Guo Houji, Pu Jian
Proctology Department, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
Graduate College, Youjiang Medical University for Nationalities, Baise, China.
Front Genet. 2021 Jun 23;12:654686. doi: 10.3389/fgene.2021.654686. eCollection 2021.
Long noncoding RNA (lncRNA) have been reported to be crucial regulators for carcinogenesis, including rectal cancer. This work aimed to explore the roles and associated mechanisms of small nucleolar RNA host gene 17 (SNHG17) in rectal cancer. A quantitative real-time polymerase chain reaction was performed to measure the expression level of SNHG17 in rectal cancer tissues and cells. Cell counting kit-8 (CCK-8) assay and flow cytometry assay were conducted to measure the biological roles of SNHG17 in rectal cancer. In addition, luciferase activity reporter assay, RNA immunoprecipitation (RIP) assay, and rescue experiments were conducted to explore the mechanisms of SNHG17 in rectal cancer. The upregulation status of SNHG17 was identified in rectal cancer tissues and cells. Functionally, knockdown the expression of SNHG17 inhibits rectal cancer cell proliferation stimulating cell apoptosis. assay showed that the knockdown of SNHG17 inhibits tumor growth. Furthermore, we showed that microRNA-361-3p (miR-361-3p) has decreased expression in tumor tissues and cells, and SNHG17 functions as a sponge for miR-361-3p. The upregulation status of stanniocalcin 2 (STC2) was also found in rectal cancer, and the knockdown of STC2 hinders cancer progression. In conclusion, lncRNA SNHG17 functions as an oncogenic lncRNA in rectal cancer by regulating the miR-361-3p/STC2 axis.
据报道，长链非编码RNA（lncRNA）是包括直肠癌在内的癌症发生的关键调节因子。这项工作旨在探讨小核仁RNA宿主基因17（SNHG17）在直肠癌中的作用及相关机制。采用定量实时聚合酶链反应来检测直肠癌组织和细胞中SNHG17的表达水平。进行细胞计数试剂盒-8（CCK-8）检测和流式细胞术检测，以测定SNHG17在直肠癌中的生物学作用。此外，进行荧光素酶活性报告基因检测、RNA免疫沉淀（RIP）检测和拯救实验，以探究SNHG17在直肠癌中的作用机制。在直肠癌组织和细胞中发现了SNHG17的上调状态。在功能上，敲低SNHG17的表达可抑制直肠癌细胞增殖并刺激细胞凋亡。检测表明，敲低SNHG17可抑制肿瘤生长。此外，我们发现微小RNA-361-3p（miR-361-3p）在肿瘤组织和细胞中的表达降低，且SNHG17作为miR-361-3p的海绵发挥作用。在直肠癌中还发现了2型鲟钙蛋白（STC2）的上调状态，敲低STC2会阻碍癌症进展。总之，lncRNA SNHG17通过调节miR-361-3p/STC2轴在直肠癌中发挥致癌lncRNA的作用。
