Cao Shuping, Li Hongxia, Li Lei
Department of Gynecology, Dongying District People's Hospital, Dongying, Shandong, People's Republic of China.
Department of Obstetrics, Dongying District People's Hospital, Dongying, Shandong, People's Republic of China.
Cancer Manag Res. 2021 Jun 23;13:4969-4978. doi: 10.2147/CMAR.S312469. eCollection 2021.
Cervical cancer is a great threat to women's health all over the world. Non-coding RNAs performed a wide range of functions. This study aimed to clarify the clinical significance and biological function of lncRNA SNHG17 and miRNA-375-3p (miR-375-3p) in cervical cancer (CC).
Blood samples from 124 CC patients and 119 healthy volunteers were collected. The relative expression of SNHG17 and miR-375-3p in CC patient serums and cells was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). The receiver operating curve (ROC) was plotted for diagnostic value estimation. The CCK-8 and transwell assay were conducted to explore the function of SNHG17 on CC cells. A luciferase reporter assay was carried out to confirm the interaction of SNHG17 and miR-375-3p. Rescue experiments were performed to verify the interaction.
SNHG17 showed an ascending expression while miR-375-3p descended in the serum of CC patients. For SNHG17 and miR-375-3p, respectively, the AUC was 0.863 and 0.869, the sensitivity was 84.7% and 75.8%, and the specificity was 78.2% and 86.6%. Knockdown of SNHG17 inhibited proliferation, migration, and invasion of CC cells. Serum SNHG17 expression was negatively correlated with miR-375-3p expression, and miR-375-3p was the target miRNA of SNHG17. Rescue experiments verified the knockdown of SNHG17 inhibited cell growth through repressing miR-375-3p expression.
SNHG17 and miR-375-3p have the potential to be diagnostic markers for CC. Overexpression of SNHG17 in CC promoted the progression of CC partly via targeting miR-375-3p, implying a novel therapeutic target for CC emerging.
宫颈癌对全球女性健康构成巨大威胁。非编码RNA发挥着广泛的功能。本研究旨在阐明长链非编码RNA SNHG17和微小RNA-375-3p（miR-375-3p）在宫颈癌（CC）中的临床意义和生物学功能。
收集124例CC患者和119名健康志愿者的血液样本。采用定量实时聚合酶链反应（qRT-PCR）评估CC患者血清和细胞中SNHG17和miR-375-3p的相对表达。绘制受试者工作特征曲线（ROC）以评估诊断价值。进行CCK-8和Transwell实验以探究SNHG17对CC细胞的作用。开展荧光素酶报告基因实验以证实SNHG17与miR-375-3p的相互作用。进行挽救实验以验证这种相互作用。
CC患者血清中SNHG17表达呈上升趋势，而miR-375-3p表达下降。SNHG17和miR-375-3p的曲线下面积（AUC）分别为0.863和0.869，灵敏度分别为84.7%和75.8%，特异性分别为78.2%和86.6%。敲低SNHG17可抑制CC细胞的增殖、迁移和侵袭。血清SNHG17表达与miR-375-3p表达呈负相关，且miR-375-3p是SNHG17的靶标微小RNA。挽救实验证实敲低SNHG17通过抑制miR-375-3p表达抑制细胞生长。
SNHG17和miR-375-3p有潜力成为CC的诊断标志物。CC中SNHG17的过表达部分通过靶向miR-375-3p促进CC进展，这意味着出现了一种新的CC治疗靶点。
