Soto-Otero R, Mendez-Alvarez E, Sierra-Paredes G, Galan-Valiente J, Aguilar-Veiga E, Sierra-Marcuño G
University of Santiago, Department of Biochemistry and Molecular Biology, School of Medicine, Spain.
Anal Biochem. 1987 Sep;165(2):331-6. doi: 10.1016/0003-2697(87)90277-6.
A precise and reproducible high-performance liquid chromatographic method for the determination of pentylenetetrazol in serum and brain tissue is described. The procedure employs reversed-phase chromatography, monitoring the eluant at 202 nm. Quantification is based on peak-height ratio of the drug to the internal standard (p-methylphenobarbital). A linear response is obtained to 100 micrograms/ml (serum) or micrograms/g (brain tissue). Within-day and between-day precision are smaller than 5%, and analytical recovery is greater than 95%. Numerous drugs tested do not interfere with the assay. The method has been used to investigate the kinetics of pentylenetetrazol distribution in serum and in discrete areas of rat brain.
本文描述了一种用于测定血清和脑组织中戊四氮的精确且可重复的高效液相色谱法。该方法采用反相色谱，在202nm处监测洗脱液。定量基于药物与内标（对甲基苯巴比妥）的峰高比。在100微克/毫升（血清）或微克/克（脑组织）范围内可获得线性响应。日内和日间精密度小于5%，分析回收率大于95%。所测试的多种药物不干扰该测定。该方法已用于研究戊四氮在血清和大鼠脑离散区域中的分布动力学。
