Alvinerie M, Sutra J F, Galtier P, Toutain P L
Laboratoire de Pharmacologie, INRA, Toulouse, France.
Ann Rech Vet. 1987;18(3):269-74.
A sensitive and specific high performance liquid chromatographic technique is described for the determination of ivermectin in milk. Milk samples (1 ml) were extracted with organic solvent (iso-octane and acetonitrile) and then chromatographed using reversed phase system. Specificity of the assay was confirmed by the use of mass spectrometry. After an subcutaneous administration of ivermectin (0.2 mg/kg) in a cow, ivermectin was detected in milk for about three weeks and over this period milk and plasma concentration were of the same order of magnitude.
描述了一种灵敏且特异的高效液相色谱技术，用于测定牛奶中的伊维菌素。取1毫升牛奶样品，用有机溶剂（异辛烷和乙腈）萃取，然后采用反相系统进行色谱分析。通过质谱法确认了该分析方法的特异性。给一头奶牛皮下注射伊维菌素（0.2毫克/千克）后，在牛奶中检测到伊维菌素约三周，在此期间牛奶和血浆中的浓度处于相同数量级。
