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产生具有抗菌、抗氧化、抗癌和抗病毒活性的次级代谢产物的海洋海绵相关链霉菌NMF6菌株的分离与鉴定

Isolation and characterization of marine sponge-associated Streptomyces sp. NMF6 strain producing secondary metabolite(s) possessing antimicrobial, antioxidant, anticancer, and antiviral activities.

作者信息

Fahmy Nayer Mohamed, Abdel-Tawab Asmaa Mohamed

机构信息

Marine Microbiology Laboratory, National Institute of Oceanography and Fisheries, Cairo, Egypt.

Marine Biotechnology and Natural Products Laboratory, National Institute of Oceanography and Fisheries, Cairo, Egypt.

出版信息

J Genet Eng Biotechnol. 2021 Jul 15;19(1):102. doi: 10.1186/s43141-021-00203-5.

DOI:10.1186/s43141-021-00203-5
PMID:34264405
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8281025/
Abstract

BACKGROUND

Actinomycetes associated with marine sponge represent a promising source of bioactive compounds. Herein, we report the isolation, identification, and bioactivity evaluation of Streptomyces sp. NMF6 associated with the marine sponge Diacarnus ardoukobae.

RESULTS

Results showed that the strain belonged to the genus Streptomyces, and it was designated as Streptomyces sp. NMF6 with the GenBank accession number MW015111. Ethyl acetate (EtOAc) extract of the strain NMF6 demonstrated a promising antimicrobial activity against Staphylococcus aureus, Enterococcus faecalis, Vibrio damsela, and Candida albicans and a strong antioxidant activity, which were confirmed by DPPH, ferric-reducing power, and phosphomolybdenum assays; results are expressed as ascorbic acid equivalents. NMF6 extract also demonstrated cytotoxicity against breast cancer cell line (MCF-7), hepatocellular carcinoma cell line (Hep-G2), and human colon carcinoma cell line (HCT-116); the selectivity index values were < 2. The extract showed promising antiviral activity against HSV-1, CoxB4, and hepatitis A viruses at concentrations that were nontoxic to the host cells, with the selectivity index values being 13.25, 9.42, and 8.25, respectively. GC-MS analysis of the extract showed the presence of 20 compounds, with bis(2-ethylhexyl) phthalate being the major component (48%).

CONCLUSIONS

Our study indicates that the marine sponge-associated Streptomyces sp. NMF6 strain is a potential source of bioactive compounds that could be developed into therapeutic agents.

摘要

背景

与海洋海绵相关的放线菌是生物活性化合物的一个有前景的来源。在此，我们报告了与海洋海绵阿杜科巴双盘海绵相关的链霉菌属菌株NMF6的分离、鉴定及生物活性评估。

结果

结果表明该菌株属于链霉菌属，被命名为链霉菌属菌株NMF6，其GenBank登录号为MW015111。菌株NMF6的乙酸乙酯提取物对金黄色葡萄球菌、粪肠球菌、美人鱼弧菌和白色念珠菌表现出有前景的抗菌活性以及较强的抗氧化活性，这通过DPPH、铁还原能力和磷钼酸测定得到证实；结果以抗坏血酸当量表示。NMF6提取物对乳腺癌细胞系（MCF - 7）、肝癌细胞系（Hep - G2）和人结肠癌细胞系（HCT - 116）也表现出细胞毒性；选择性指数值<2。该提取物在对宿主细胞无毒的浓度下对单纯疱疹病毒1型（HSV - 1）、柯萨奇病毒B4（CoxB4）和甲型肝炎病毒表现出有前景的抗病毒活性，选择性指数值分别为13.25、9.42和8.25。提取物的气相色谱 - 质谱分析表明存在20种化合物，邻苯二甲酸二（2 - 乙基己基）酯为主要成分（48%）。

结论

我们的研究表明，与海洋海绵相关的链霉菌属菌株NMF6是一种潜在的生物活性化合物来源，可开发成治疗药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/06f35f84d863/43141_2021_203_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/44b188930671/43141_2021_203_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/ecb990e55521/43141_2021_203_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/1e079f41820a/43141_2021_203_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/dd30339ef54a/43141_2021_203_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/3f41169d23fc/43141_2021_203_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/9892fbafdf79/43141_2021_203_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/e95a29ef68ff/43141_2021_203_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/06f35f84d863/43141_2021_203_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/44b188930671/43141_2021_203_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/ecb990e55521/43141_2021_203_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/1e079f41820a/43141_2021_203_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/dd30339ef54a/43141_2021_203_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/3f41169d23fc/43141_2021_203_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/9892fbafdf79/43141_2021_203_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/e95a29ef68ff/43141_2021_203_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27a8/8282828/06f35f84d863/43141_2021_203_Fig8_HTML.jpg

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