van der Meer J W, Bulterman D, van Zwet T L, Elzenga-Claasen I, van Furth R
J Exp Med. 1978 Jan 1;147(1):271-6. doi: 10.1084/jem.147.1.271.
A method is described for the culture of mononuclear phagocytes in suspension by incubation on a Teflon film to which the cells do not adhere. The characteristics of peritoneal macrophages, bone marrow mononuclear phagocytes, macrophage cell lines, and fibroblasts cultured in this way are similar to those observed after culture on glass or plastic surfaces. Culture of mononuclear phagocytes in Teflon film dishes has three important advantages: the cells can be easily harvested without damage, recovery is almost complete, and the cells are not functionally impaired. Thus, this method makes it possible to use cultured mononuclear phagocytes for many studied that could previously only be done in freshly collected cells.
本文描述了一种通过在细胞不会黏附的特氟龙薄膜上孵育来悬浮培养单核吞噬细胞的方法。以这种方式培养的腹膜巨噬细胞、骨髓单核吞噬细胞、巨噬细胞系和成纤维细胞的特征，与在玻璃或塑料表面培养后观察到的特征相似。在特氟龙薄膜培养皿中培养单核吞噬细胞有三个重要优点：细胞可以轻松收获而不被破坏，回收率几乎是100%，并且细胞功能不会受损。因此，这种方法使得培养的单核吞噬细胞能够用于许多以前只能用新鲜采集的细胞进行的研究。
