College of Horticulture, Hunan Agricultural University, Changsha, China.
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Shenzhen Key Laboratory of Agricultural Synthetic Biology, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
Nat Plants. 2021 Aug;7(8):1026-1036. doi: 10.1038/s41477-021-00963-5. Epub 2021 Jul 15.
The ancient gymnosperm genus Taxus is the exclusive source of the anticancer drug paclitaxel, yet no reference genome sequences are available for comprehensively elucidating the paclitaxel biosynthesis pathway. We have completed a chromosome-level genome of Taxus chinensis var. mairei with a total length of 10.23 gigabases. Taxus shared an ancestral whole-genome duplication with the coniferophyte lineage and underwent distinct transposon evolution. We discovered a unique physical and functional grouping of CYP725As (cytochrome P450) in the Taxus genome for paclitaxel biosynthesis. We also identified a gene cluster for taxadiene biosynthesis, which was formed mainly by gene duplications. This study will facilitate the elucidation of paclitaxel biosynthesis and unleash the biotechnological potential of Taxus.
古老的裸子植物红豆杉属是抗癌药物紫杉醇的唯一来源，但目前还没有参考基因组序列可用于全面阐明紫杉醇的生物合成途径。我们已经完成了 Taxus chinensis var. mairei 的染色体水平基因组测序，其总长度为 102.3 亿碱基对。红豆杉属与针叶树类群共享一个祖先全基因组加倍事件，并经历了独特的转座子进化。我们在红豆杉基因组中发现了一个独特的 CYP725A（细胞色素 P450）物理和功能分组，用于紫杉醇的生物合成。我们还鉴定了一个 taxadiene 生物合成基因簇，它主要是由基因复制形成的。这项研究将有助于阐明紫杉醇的生物合成，并释放红豆杉的生物技术潜力。
