Schellenberg F, Weill J
Biochemistry Lab., Medical School, University Hospital, Tours, France.
Alcohol Alcohol Suppl. 1987;1:624-9.
We developed a method for the quantification of the different fractions of serum transferrin, based on an isoelectric focusing followed by immunofixation. We employed this technique to study whether or not the pattern of the serum transferrin was altered in the serum of drinkers. We established an increase of the fraction that focused at 5.7 (Tf5.7) and a concomittant decrease of the fraction that focused at 5.4 (Tf5.4) in the sera of drinkers. The separation between drinkers and controls was enhanced when using the ratio Tf5.7/Tf5.4, called Tf index. The predictive value of this test was 0.88, which means that 88% of the subjects were correctly classified as heavy drinkers or not.
我们开发了一种基于等电聚焦后免疫固定法对血清转铁蛋白不同组分进行定量的方法。我们运用该技术研究饮酒者血清中转铁蛋白模式是否发生改变。我们发现饮酒者血清中聚焦于5.7的组分（Tf5.7）增加，而聚焦于5.4的组分（Tf5.4）相应减少。使用Tf5.7/Tf5.4的比值（称为Tf指数）时，饮酒者与对照组之间的区分更为明显。该检测的预测价值为0.88，这意味着88%的受试者被正确分类为重度饮酒者或非重度饮酒者。
