Vesterberg O, Petrén S, Schmidt D
Clin Chim Acta. 1984 Aug 15;141(1):33-9. doi: 10.1016/0009-8981(84)90164-5.
Isoelectric focusing in agarose gel has been used for separation of different molecular forms of transferrin (Tf) in sera and plasma from alcoholics and controls. One special form of transferrin with isoelectric point 5.7 (= Tf5.7) was quantified by using zone immunoelectrophoresis assay (ZIA). This method was also used for quantification of total transferrin (Tftot). Significant differences in the ratio Tf5.7/Tftot between the groups were obtained and the diagnostic sensitivities, specificities and predictive values of positive tests were 100, 97, 93.8%, respectively. Thus the results showed that this principle seems to be much better for detection of alcoholic abuse than currently used assays as gamma-glutamyltransferase, etc. Samples taken on days following hospitalization showed that the Tf5.7 values decreased over a two-week period of abstinence. Possible explanations for the abnormal quotient after ethanol abuse are discussed.
琼脂糖凝胶等电聚焦已用于分离酗酒者和对照组血清及血浆中不同分子形式的转铁蛋白（Tf）。采用区带免疫电泳法（ZIA）对一种等电点为5.7的特殊转铁蛋白形式（= Tf5.7）进行定量。该方法也用于总转铁蛋白（Tftot）的定量。两组之间Tf5.7/Tftot比值存在显著差异，阳性检测的诊断敏感性、特异性和预测值分别为100%、97%、93.8%。因此，结果表明，与目前使用的γ-谷氨酰转移酶等检测方法相比，这一原理在检测酒精滥用方面似乎要好得多。住院后几天采集的样本显示，在戒酒两周期间Tf5.7值下降。文中讨论了乙醇滥用后异常商数的可能解释。
