Dumon M F, Nau A, Hervouet M, Paccalin J, Clerc M
Laboratoire Central de Biochimie, Hôpital Saint-André, Bordeaux, France.
Clin Biochem. 1996 Dec;29(6):549-53. doi: 10.1016/s0009-9120(96)00102-6.
A new simplified method for detection and quantitation of disialontransferrin in serum is described.
The method is based on polyacrylamide gel isoelectric focusing, direct immunofixation with a specific antibody, and measurement by computerized scanning densitometry. Disialotransferrin levels were determined in 24 teetotallers and 34 alcoholics at 3 moments during detoxification. Three groups of drinkers were arranged: group 1 (without), group 2 (with light), and group 3 (with severe hepatitis).
The metho showed very good reproducibility and accuracy with a coefficient of variation between 5 to 8%. Alcoholic patients could be clearly separated from teetotallers, with a specificity of 100% and a sensitivity of 94%. After 12 days of alcohol withdrawal, disialotransferrin values declined in alcoholics but remained slightly high. They were not influenced by the severity of liver disease. No significant difference was found between the 3 groups.
An easy-to-perform, sensitive, and inexpensive method has been developed to quantify disialotransferrin that can be used by laboratories almost everywhere.
描述一种检测和定量血清中双唾液酸转铁蛋白的新简化方法。
该方法基于聚丙烯酰胺凝胶等电聚焦、用特异性抗体进行直接免疫固定以及通过计算机扫描密度测定法进行测量。在戒酒过程中的3个时间点，对24名戒酒者和34名酗酒者的双唾液酸转铁蛋白水平进行了测定。将饮酒者分为三组：第1组（无肝病）、第2组（轻度肝病）和第3组（重度肝炎）。
该方法显示出非常好的重现性和准确性，变异系数在5%至8%之间。酗酒患者与戒酒者能够明显区分，特异性为100%，敏感性为94%。戒酒12天后，酗酒者的双唾液酸转铁蛋白值下降，但仍略高。它们不受肝病严重程度的影响。三组之间未发现显著差异。
已开发出一种易于操作、灵敏且廉价的方法来定量双唾液酸转铁蛋白，几乎任何地方的实验室都可使用。
