Schellenberg F, Weill J
Drug Alcohol Depend. 1987 Mar;19(2):181-91. doi: 10.1016/0376-8716(87)90056-1.
Different molecular forms of transferrin (Tf) have been separated in sera from alcoholics and controls by isoelectric focusing on ultrathin polyacrylamide gels using a semi narrow pH range. The main fraction focused at pH 5.4 (Tf5.4) and minor components appeared among controls at pH 5.7 (Tf5.7). This fraction was increased among alcoholics and was sometimes accompanied by a fraction focusing at pH 5.9 (Tf5.9). Fractions were quantified by densitometry following immunofixation. Significant differences in the ratios Tf5.4/Tf and Tf5.7/Tf between the groups were obtained, but no difference could be determined in the total Tf. We used the ratio Tf5.7/Tf5.4 called Tf index to distinguish alcoholics from controls. The sensitivity, the specificity and the global predictive value of this test were 0.82, 1 and 0.88 respectively. These results seems to indicate that the Tf index is more reliable for the detection of alcohol abuse than liver enzymes such as gammaglutamyl transferase (gamma GT) or glutamate dehydrogenase (GDH).
通过在超薄聚丙烯酰胺凝胶上使用半窄pH范围进行等电聚焦，已将酒精性肝病患者和对照组血清中的转铁蛋白（Tf）的不同分子形式分离出来。主要部分聚焦在pH 5.4（Tf5.4），在对照组中，次要成分出现在pH 5.7（Tf5.7）。该部分在酒精性肝病患者中增加，有时还伴有聚焦在pH 5.9的部分（Tf5.9）。免疫固定后通过光密度法对各部分进行定量。两组之间Tf5.4/Tf和Tf5.7/Tf的比值存在显著差异，但总Tf未发现差异。我们使用Tf5.7/Tf5.4的比值（称为Tf指数）来区分酒精性肝病患者和对照组。该检测的敏感性、特异性和总体预测值分别为0.82、1和0.88。这些结果似乎表明，Tf指数在检测酒精滥用方面比γ-谷氨酰转移酶（γ-GT）或谷氨酸脱氢酶（GDH）等肝脏酶更可靠。
