Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, India.
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, JNT University Hyderabad, Kukatpally, Hyderabad 500085, India.
Syst Appl Microbiol. 2021 Jul;44(4):126229. doi: 10.1016/j.syapm.2021.126229. Epub 2021 Jul 9.
Four strains of Planctomycetes, (JC636, JC649, JC650, JC657) which are all salt and alkali tolerant, pink coloured, with spherical to oval shaped, Gram-stain-negative, non-motile cells were isolated from different regions of Chilika lagoon, India. All strains have obligate requirement for N-acetylglucosamine (NAG) and share highest 16S rRNA gene sequence identity with members of the genus Tautonia (<95%) of the family Isosphaeraceae. The 16S rRNA gene sequence identity between strains was >99.5%. Respiratory quinone for all the strains was MK6. Major fatty acids of all the strains were Cω9c, C and C. Major polar lipid of the strain JC650 was phosphatidylethanolamine, while, phosphatidylcholine and phosphatidylglycerol for strain JC657. Spermidine was the only common polyamine for all the four strains. Strains JC657, JC636 and JC649 shared highest phenotypic similarity along with 100% 16S rRNA gene sequence identity. Strains JC657, JC636 and JC649 differed from strain JC650 phenotypically, chemotaxonomically and genotypically, thus belong to a different species. The genomic size of strain JC650 and JC657 are 7.06 Mb and 6.96 Mb with DNA G + C content of 63.9 and 62.7 mol%, respectively. Based on phylogenetic, genomic (ANI, AAI, POCP, dDDH), chemotaxonomic, physiological and biochemical characteristics, we conclude that strains JC650 and JC657 (together with strains JC636, JC649) belong to the genus Tautonia and constitute two novel species for which we propose the names Tautonia marina sp. nov., and Tautonia rosea sp. nov., respectively. These two novel species are represented by the type strains JC650 (=KCTC 72177 = NBRC 113885) and JC657 (=KCTC 72597 = NBRC 113883) respectively.
从印度奇拉卡湖的不同地区分离到 4 株盐耐受和碱耐受的浮霉状菌（JC636、JC649、JC650 和 JC657），它们均为粉红色、球形到椭圆形、革兰氏阴性、非运动细胞。所有菌株都需要 N-乙酰葡萄糖胺（NAG），与螺旋体科 Isosphaeraceae 属的 Tautonia 属成员的 16S rRNA 基因序列同源性最高（<95%）。菌株间的 16S rRNA 基因序列同一性>99.5%。所有菌株的呼吸醌均为 MK6。所有菌株的主要脂肪酸均为 Cω9c、C 和 C。菌株 JC650 的主要极性脂为磷脂酰乙醇胺，而菌株 JC657 的主要极性脂为磷脂胆碱和磷脂甘油。精胺是这 4 株菌共有的唯一多胺。菌株 JC657、JC636 和 JC649 的表型相似性最高，16S rRNA 基因序列同一性为 100%。菌株 JC657、JC636 和 JC649 在表型、化学分类和基因型上与菌株 JC650 不同，因此属于不同的种。菌株 JC650 和 JC657 的基因组大小分别为 7.06 Mb 和 6.96 Mb，GC 含量分别为 63.9%和 62.7%mol%。基于系统发育、基因组（ANI、AAI、POCP、dDDH）、化学分类、生理生化特性，我们得出结论，菌株 JC650 和 JC657（与菌株 JC636、JC649 一起）属于 Tautonia 属，构成了两个新种，分别命名为 Tautonia marina sp. nov. 和 Tautonia rosea sp. nov.。这两个新种分别由模式菌株 JC650（=KCTC 72177=NRBC 113885）和 JC657（=KCTC 72597=NRBC 113883）代表。
