Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, India.
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, JNT University Hyderabad, Kukatpally, Hyderabad-500085, India.
Int J Syst Evol Microbiol. 2020 Jun;70(6):3647-3655. doi: 10.1099/ijsem.0.004211. Epub 2020 May 5.
A Gram-stain-negative, aerobic, non-motile, salt- and alkali-tolerant, pear to oval shaped, rosette-forming, white coloured, bacterium, designated as strain JC646, was isolated from a sediment sample collected from Chilika lagoon, India. Strain JC646 reproduced through budding, grew well at up to pH 9.0 and tolerated up to 7 % NaCl. Strain JC 646 utilized α-d-glucose, fumarate, lactose, sucrose, fructose, d-galactose, mannose, maltose and d-xylose as carbon sources. Peptone, l-isoleucine, l-serine, l-lysine, l-glutamic acid, l-aspartic acid, dl-threonine and l-glycine were used by the strain as nitrogen sources for growth. The respiratory quinone was MK6. Major fatty acids were C ω7/C ω6 and C. The polar lipids of strain JC646 comprised phosphatidyl-dimethylethanolamine, phosphatidylcholine, diphosphatidylglycerol, an unidentified amino lipid and two unidentified lipids. Strain JC646 had highest (97.3 %) 16S rRNA gene sequence identity to the only species of the genus , DSM 8797. The genome of strain JC646 was 7.64 Mbp with a DNA G+C content of 53.2 mol%. For the resolution of the phylogenetic congruence of the novel strain, the phylogeny was also reconstructed with the sequences of 92 housekeeping genes. Based on phylogenetic analyses, digital DNA-DNA hybridization (19.0 %), genome average nucleotide identity (74.5 %) and average amino acid identity/percentageof conserved proteins (77 %) results, chemotaxonomic characteristics, and differential physiological properties, strain JC646 is recognized as representing a new species of the genus , for which we propose the name sp. nov. The type strain is JC646 (=KCTC 72175=NBRC 113881).
一株革兰氏阴性、需氧、不运动、耐盐和耐碱、呈梨形到椭圆形、形成玫瑰花结、白色的细菌，被命名为 JC646 菌株，从印度奇拉卡泻湖的沉积物样本中分离得到。JC646 菌株通过出芽繁殖，在 pH 值高达 9.0 时生长良好，可耐受高达 7%的 NaCl。JC646 菌株利用 α-d-葡萄糖、富马酸、乳糖、蔗糖、果糖、d-半乳糖、甘露糖、麦芽糖和 d-木糖作为碳源。该菌株将蛋白胨、l-异亮氨酸、l-丝氨酸、l-赖氨酸、l-谷氨酸、l-天冬氨酸、dl-苏氨酸和 l-甘氨酸用作生长的氮源。呼吸醌为 MK6。主要脂肪酸为 Cw7/Cw6 和 C。JC646 菌株的极性脂包括磷脂酰二甲乙醇胺、磷脂酰胆碱、双磷脂酰甘油、一种未鉴定的氨基酸脂和两种未鉴定的脂。JC646 菌株与属内唯一的种 DSM 8797 的 16S rRNA 基因序列相似性最高（97.3%）。JC646 菌株的基因组大小为 7.64 Mbp，GC 含量为 53.2 mol%。为了解决新菌株的系统发育一致性问题，还使用了 92 个看家基因的序列重建了系统发育。基于系统发育分析、数字 DNA-DNA 杂交（19.0%）、全基因组平均核苷酸同一性（74.5%）和平均氨基酸同一性/保守蛋白百分比（77%）结果、化学生态特征以及差异生理特性，JC646 菌株被认为代表了属的一个新种，我们建议将其命名为 sp. nov。模式菌株为 JC646（=KCTC 72175=NBRC 113881）。
