Lhingjakim Khongsai L, Smita Nandardhane, Kumar Gaurav, Jagadeeshwari Uppada, Ahamad Shabbir, Sasikala Chintalapati, Ramana Chintalapati Venkata
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500046, India.
Department of Microbiology, School of Sciences, ITM (SLS) Baroda University, Halol, Vadodara, Gujarat, 391510, India.
Antonie Van Leeuwenhoek. 2022 Aug;115(8):1073-1084. doi: 10.1007/s10482-022-01758-0. Epub 2022 Jul 2.
Two axenic cultures of Planctomycetota were isolated from distinct geographical locations of India. Strain JC665 was isolated from a rhizosphere soil of Loktak lake, Manipur, whereas strain JC747 was isolated from a soil sediment at Pallikkara village, Kerala, India. The two closely related strains shared the highest 16S rRNA gene sequence identity (94.6%) with Paludisphaera borealis PX4, while the 16S rRNA gene sequence identity between both strains was 100%. Both strains grow aerobically, stain Gram negative, colonies are light pink-coloured, cells are non-motile, spherical to oval-shaped and tolerate NaCl up to 2% (w/v). While strain JC665 grows well up to pH 9.0, strain JC747 grows only up to pH 8.0. The respiratory quinone in both strains is MK-6. C, Cω9c and C are the major fatty acids. Phosphatidylcholine, two unidentified glycolipids, seven unidentified lipids and two unidentified phospholipids made up the polar lipid composition of both strains. Both strains have genome sizes of about 8.0 Mb and a DNA G + C content of 66.4 mol%. Both strains contain genes coding for enzymes putatively involved in the production of lycopene-related carotenoids. The phylogenetic position together with the results of the analysis of morphological, physiological and genomic features support the classification of strain JC665 as a new species of the genus Paludisphaera, for which we propose the name Paludisphaera rhizosphaerae sp. nov. Strain JC665 (= KCTC 72671 = NBRC 114305) and JC747 are the type and non-type strain of the new species, respectively.
从印度不同地理位置分离出了两种浮霉菌门的无菌培养物。菌株JC665是从印度曼尼普尔邦洛克塔克湖的根际土壤中分离出来的，而菌株JC747是从印度喀拉拉邦帕利卡拉村的土壤沉积物中分离出来的。这两个密切相关的菌株与北方帕鲁迪斯菌PX4的16S rRNA基因序列具有最高的同一性（94.6%），而这两个菌株之间的16S rRNA基因序列同一性为100%。两个菌株均为好氧生长，革兰氏染色阴性，菌落呈浅粉色，细胞无运动性，球形至椭圆形，可耐受高达2%（w/v）的NaCl。菌株JC665在pH 9.0时生长良好，而菌株JC747仅在pH 8.0时生长。两个菌株中的呼吸醌均为MK-6。C、Cω9c和C是主要脂肪酸。磷脂酰胆碱、两种未鉴定的糖脂、七种未鉴定的脂质和两种未鉴定的磷脂构成了两个菌株的极性脂质组成。两个菌株的基因组大小约为8.0 Mb，DNA G+C含量为66.4 mol%。两个菌株都含有编码可能参与番茄红素相关类胡萝卜素生产的酶的基因。系统发育位置以及形态、生理和基因组特征分析结果支持将菌株JC665分类为帕鲁迪斯菌属的一个新物种，我们为此提议将其命名为根际帕鲁迪斯菌（Paludisphaera rhizosphaerae sp. nov.）。菌株JC665（=KCTC 72671=NBRC 114305）和JC747分别是新物种的模式菌株和非模式菌株。
