Wang Jian, Ruan Chu-Jin, Song Lei, Li Ang, Zhu Ya-Xin, Zheng Xiao-Wei, Wang Li, Lu Zu-Jun, Huang Ying, Du Wenbin, Zhou Yuguang, Huang Li, Dai Xin
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Present address: China Agricultural University, Beijing, PR China.
Int J Syst Evol Microbiol. 2020 Jul;70(7):4384-4389. doi: 10.1099/ijsem.0.004301.
A Gram-stain-negative, stalked, oval-shaped and budding bacterial strain, designated E7, was isolated from a deep-sea water sample collected from the Northwest Indian Ocean. The novel strain was strictly aerobic, and catalase- and oxidase-positive. It grew at 6-40 °C (optimum 30 °C) and pH 5.5-8.0 (optimum pH 7.0-7.5). The strain required 0.5-9.0 % (w/v) NaCl (optimum 3.0-5.0 %) for growth. Aesculin, starch, pectin and Tween 20 were hydrolysed. Based on 16S rRNA gene sequence analysis, strain E7 showed the highest similarity with DSM 8797 (97.5 %). The average nucleotide identity and DNA-DNA hybridization values between strain E7 and DSM 8797 were 78.0 and 19.3 %, respectively. The predominant cellular fatty acids of strain E7 were C and summed feature 3 (comprising Cω7 and/or Cω6). The major respiratory quinone was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PMME), phosphatidyldimethylethanolamine (PDME), phosphatidylcholine (PC) and diphosphatidylglycerol (DPG). The genomic DNA G+C content of strain E7 was 52.8 mol%. On the basis of phylogenetic inference and phenotypic characteristics, it is proposed that strain E7 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is E7 (=CGMCC 1.16119=KCTC 72737).
从西北印度洋采集的深海水样中分离出一株革兰氏染色阴性、具柄、椭圆形且能出芽的细菌菌株，命名为E7。该新菌株为严格需氧菌，过氧化氢酶和氧化酶呈阳性。它在6 - 40°C（最适温度30°C）和pH 5.5 - 8.0（最适pH 7.0 - 7.5）条件下生长。该菌株生长需要0.5 - 9.0%（w/v）NaCl（最适3.0 - 5.0%）。七叶苷、淀粉、果胶和吐温20可被水解。基于16S rRNA基因序列分析，菌株E7与DSM 8797的相似性最高（97.5%）。菌株E7与DSM 8797之间的平均核苷酸同一性和DNA - DNA杂交值分别为78.0%和19.3%。菌株E7的主要细胞脂肪酸为C和总和特征3（包含Cω7和/或Cω6）。主要呼吸醌为甲基萘醌 - 6（MK - 6），主要极性脂为磷脂酰乙醇胺（PE）、磷脂酰单甲基乙醇胺（PMME）、磷脂酰二甲基乙醇胺（PDME）、磷脂酰胆碱（PC）和双磷脂酰甘油（DPG）。菌株E7的基因组DNA G + C含量为52.8 mol%。基于系统发育推断和表型特征，建议菌株E7代表一个新的属种，为此提出新种名为 sp. nov.。模式菌株为E7（=CGMCC 1.16119 = KCTC 72737）。
