The Role of Nitric Oxide-Induced in Growth and Disease Resistance in .

Nitric oxide (NO) is a signaling molecule that regulates various processes, including plant growth and development, immunity, and environmental interactions. Using high throughput RNA-seq data, we explored the role of the NO-induced gene in plant growth and defense using functional genomics. The mutant and wild-types were challenged with either oxidative (HO, MV) or nitro-oxidative (CySNO, GSNO) stress conditions, and the phenotypic results showed that gene differentially regulates cotyledon development frequency (CDF) as well as the root and shoot lengths of the plants. To investigate whether plays a role in plant basal or ()-mediated defense, the plants were challenged with either virulent or avirulent strains of pathovar tomato (Pst) DC3000. The line showed a susceptible phenotype, higher pathogen growth, and highly reduced transcript accumulation of and genes. These results suggested that positively regulates plant basal defense. Furthermore, after the inoculation of with avirulent Pst (DC3000), the expressions of the and genes decreased, suggesting a positive role in -mediated resistance in protecting the plant from further spread of disease. We also investigated the role of in systemic acquired resistance (SAR), and the results showed that positively regulates SAR, as the mutant line has significantly ( ≤ 0.05) lower transcript accumulation of , and genes. Overall, these results indicate that the NO-induced gene differentially regulates plant growth and positively regulates plant basal defense, -mediated resistance, and SAR.