Niyoifasha Chrizostom Julius, Borena Birhanu Miressa, Ukob Irasapa Tanimu, Minh Phan Ngoc, Al Azzawi Tiba Nazar Ibrahim, Imran Muhammad, Ali Sajid, Inthavong Anousone, Mun Bong-Gyu, Lee In-Jung, Khan Murtaza, Yun Byung-Wook
Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
Biosafety Division, National Institute of Agriculture Science, Rural Development Administration, Jeonju 55365, Republic of Korea.
Plants (Basel). 2023 Mar 13;12(6):1299. doi: 10.3390/plants12061299.
The cultivation of rice is widespread worldwide, but its growth and productivity are hampered by heavy metals stress. However, sodium nitroprusside (SNP), a nitric oxide donor, has been found to be effective for imparting heavy metals stress tolerance to plants. Therefore, the current study evaluated the role of exogenously applied SNP in improving plant growth and development under Hg, Cr, Cu, and Zn stress. For this purpose, heavy metals stress was induced via the application of 1 mM mercury (Hg), chromium (Cr), copper (Cu), and zinc (Zn). To reverse the toxic effects of heavy metals stress, 0.1 mM SNP was administrated via the root zone. The results revealed that the said heavy metals significantly reduced the chlorophyll contents (SPAD), chlorophyll a and b, and protein contents. However, SNP treatment significantly reduced the toxic effects of the said heavy metals on chlorophyll (SPAD), chlorophyll a and b, and protein contents. In addition, the results also revealed that heavy metals significantly increased the production of superoxide anion (SOA), hydrogen peroxide (HO), malondialdehyde (MDA), and electrolyte leakage (EL). However, SNP administration significantly reduced the production of SOA, HO, MDA, and EL in response to the said heavy metals. Furthermore, to cope with the said heavy metals stress, SNP administration significantly enhanced the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and polyphenol peroxidase (PPO). Furthermore, in response to the said heavy metals, SNP application also upregulated the transcript accumulation of , , , , , and . Therefore, SNP can be used as a regulator to improve the heavy metals tolerance of rice in heavy-metals-affected areas.
水稻种植在全球范围内广泛分布，但其生长和生产力受到重金属胁迫的阻碍。然而，已发现一氧化氮供体硝普钠（SNP）可有效赋予植物重金属胁迫耐受性。因此，本研究评估了外源施加SNP在汞、铬、铜和锌胁迫下对植物生长发育的作用。为此，通过施加1 mM汞（Hg）、铬（Cr）、铜（Cu）和锌（Zn）诱导重金属胁迫。为逆转重金属胁迫的毒性作用，通过根区施用0.1 mM SNP。结果表明，上述重金属显著降低了叶绿素含量（SPAD）、叶绿素a和b以及蛋白质含量。然而，SNP处理显著降低了上述重金属对叶绿素（SPAD）、叶绿素a和b以及蛋白质含量的毒性作用。此外，结果还表明，重金属显著增加了超氧阴离子（SOA）、过氧化氢（HO）、丙二醛（MDA）和电解质渗漏（EL）的产生。然而，施用SNP显著降低了响应上述重金属时SOA、HO、MDA和EL的产生。此外，为应对上述重金属胁迫，施用SNP显著增强了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）和多酚氧化酶（PPO）的活性。此外，响应上述重金属，施用SNP还上调了 、 、 、 、 和 的转录积累。因此，SNP可用作调节剂，以提高重金属污染地区水稻对重金属的耐受性。
