绿原酸对紫外线诱导的成纤维细胞的抗炎和抗衰老特性。
Anti-inflammatory and antiaging properties of chlorogenic acid on UV-induced fibroblast cell.
作者信息
Girsang Ermi, Ginting Chrismis N, Lister I Nyoman Ehrich, Gunawan Kamila Yashfa, Widowati Wahyu
机构信息
Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia.
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia.
出版信息
PeerJ. 2021 Jul 7;9:e11419. doi: 10.7717/peerj.11419. eCollection 2021.
BACKGROUND
Skin aging is the most common dermatological problem caused by intrinsic and extrinsic factor, such as exposure to (ultraviolet) UV rays. Chlorogenic acid (CA) is a phenolic compound which is known for its antioxidant properties against oxidative stress.
OBJECTIVE
This study investigates the antiaging and anti-inflammatory properties of CA on UV-induced skin fibroblast cells.
METHODS
Anti-inflammatory properties of CA were assessed by measuring inflammatory-related proteins IL-1β and TNF-α, while antiaging properties of CA were assessed by measuring reactive oxygen species (ROS), apoptosis, live and necrotic cells, and COL-3 gene expression level.
RESULTS
Treating UV-induced skin fibroblast cells with CA decreased the level of ROS, IL-1β, TNF-α, apoptotic cells, and necrotic cells and increased live cells and COL-3 gene expression.
CONCLUSION
CA has the potential as the protective compound against inflammation and aging by decreasing the level ROS, pro-inflammatory cytokines IL-1β and TNF-α, apoptotic cells, and necrotic cells and by increasing live cells and COL-3 gene expression.
背景
皮肤老化是由内在和外在因素引起的最常见的皮肤病学问题，如暴露于（紫外线）UV射线。绿原酸（CA）是一种酚类化合物，以其对抗氧化应激的抗氧化特性而闻名。
目的
本研究调查CA对紫外线诱导的皮肤成纤维细胞的抗老化和抗炎特性。
方法
通过测量炎症相关蛋白IL-1β和TNF-α评估CA的抗炎特性，而通过测量活性氧（ROS）、细胞凋亡、活细胞和坏死细胞以及COL-3基因表达水平评估CA的抗老化特性。
结果
用CA处理紫外线诱导的皮肤成纤维细胞可降低ROS、IL-1β、TNF-α、凋亡细胞和坏死细胞的水平，并增加活细胞和COL-3基因表达。
结论
CA具有作为抗炎和抗老化保护化合物的潜力，可通过降低ROS、促炎细胞因子IL-1β和TNF-α、凋亡细胞和坏死细胞的水平以及增加活细胞和COL-3基因表达来实现。
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