Tao R V, Lee B C, Hsieh T C, Laine R A
Department of Ophthalmology, Lions of Illinois Eye Research Institute, University of Illinois College of Medicine, Chicago 60612.
Curr Eye Res. 1987 Dec;6(12):1361-7. doi: 10.3109/02713688709044499.
Human cataractous glycosphingolipids were isolated and purified according to previously established procedures. Fatty acid analyses of the purified glycosphingolipid revealed the presence of a significant amount of an odd-chain fatty acid. This was confirmed by methane (CH4) chemical ionization mass spectrometry, which showed characteristic ions at m/z 397, 425, 437, and 365. These ions facilitated the determination of molecular weight and the assignment of C25:0 (n-pentacosanoic acid) to this fatty acid in question. This may be the first time that such an unusual distribution of a single odd-chain fatty acid has been shown to occur in glycosphingolipid from any tissue.
按照先前确立的程序分离并纯化了人白内障糖鞘脂。对纯化后的糖鞘脂进行脂肪酸分析，结果显示存在大量奇数链脂肪酸。这一点通过甲烷（CH4）化学电离质谱法得到了证实，该方法在质荷比为397、425、437和365处显示出特征离子。这些离子有助于确定分子量，并将C25:0（正二十五烷酸）归属于所讨论的这种脂肪酸。这可能是首次表明在任何组织的糖鞘脂中出现单一奇数链脂肪酸如此异常的分布情况。
