Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland; Institute of Chemical Sciences and Engineering, School of Basic Sciences, EPFL, 1015, Lausanne, Switzerland.
Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
Biomaterials. 2021 Sep;276:121020. doi: 10.1016/j.biomaterials.2021.121020. Epub 2021 Jul 10.
Organoids are a new class of biological model systems that have garnered significant interest in the life sciences. When provided with the proper 3D matrix and biochemical factors, stem cells can self-organize and form tissue-specific organoids. Thus far, there has been a substantial effort to identify soluble niche components essential for organoid culture; however, the role of the solid extracellular matrix (ECM) as an essential element of the niche is still largely lacking. In this review, we discuss the importance of the ECM in intestinal, hepatic, and pancreatic organoid culture and how biomaterial-based approaches can be used to probe different ECM properties required for more physiologically and translationally relevant organoid models.
类器官是一类新的生物模型系统，在生命科学领域引起了广泛关注。当提供适当的 3D 基质和生化因子时，干细胞可以自我组织并形成组织特异性类器官。到目前为止，人们已经做出了巨大的努力来确定对类器官培养至关重要的可溶性生态位成分；然而，固体细胞外基质 (ECM) 作为生态位的重要组成部分的作用在很大程度上仍然缺失。在这篇综述中，我们讨论了 ECM 在肠道、肝脏和胰腺类器官培养中的重要性，以及如何使用基于生物材料的方法来研究更接近生理和转化相关的类器官模型所需的不同 ECM 特性。
