Akinlolu A A, Ameen M O, Oyewopo A O, Kadir R E, Ahialaka O, Tijani S, Ogungbesan O, Bebeyi R, Adebayo S, Amoo T, Abdulazeez M
Department of Anatomy, Faculty of Basic Medical Sciences, Olabisi Onabanjo University, Ogun State, Nigeria.
Department of Chemistry, Faculty of Physical Sciences, University of Ilorin, Ilorin, Kwara State, Nigeria.
Int J Health Sci (Qassim). 2021 Jul-Aug;15(4):20-28.
Lead poisoning accounts for about 0.6% of global burden of disease. Lead-induced toxicity is through confinement of oxidative stress in affected organs. We evaluated the effects of MLF1 (extracted from leaves) and AMF1 (extracted from leaves) on lipid peroxidation and immunomodulations of Melatonin, tumor necrosis factor-alpha (TNF-α), and p53 proteins in lead acetate (LA)-induced toxicity in rats.
Sixty adult female rats were randomly divided into 12 groups (n = 5). Groups 1 and 2 received physiological saline and 100 mg/kg bodyweight of LA, respectively, for 5 weeks. Groups 3-6 received 100 mg/kg bodyweight LA for 2 weeks, followed by treatments with 7.5 and 15 mg/kg bodyweight of MLF1, and 7.5 and 10 mg/kg bodyweight of AMF1, respectively, for 3 weeks. Groups 7-10 received 7.5 and 15 mg/kg bodyweight of MLF1, 7.5 and 10 mg/kg bodyweight of AMF1, respectively, for 5 weeks. Groups 11-12 received co-administrations of 100 mg/kg bodyweight LA with 15 mg/kg bodyweight MLF1 and 10 mg/kg bodyweight of AMF1, respectively, for 5 weeks. Drugs and extracts were administered orally. Consequently, liver histopathology (Hematoxylin and Eosin), sera Melatonin, and TNF-α (enzyme-linked immunosorbent assay [ELISA]) levels were evaluated. Malondialdehyde (MDA) (thiobarbituric acid assay) and p53 (ELISA) levels were evaluated in liver homogenates. Data were statistically analyzed (P ≤ 0.05).
Results showed normal liver histology in all Groups. Statistical analyses showed significant (P ≤ 0.05) and non-significant decreased levels (P ≥ 0.05) of MDA, TNF-α and p53 in Groups 3-12, compared with Group 2. Furthermore, results showed significant (P ≤ 0.05) and non-significant increased Melatonin levels (P ≥ 0.05) in Groups 4-12 compared with Group 2.
This study confirmed that MLF1 and AMF1 confer a degree of antioxidant, anticancer and hepato-protetive potentials against LA-induced toxicity in rats.
铅中毒约占全球疾病负担的0.6%。铅诱导的毒性是通过在受影响器官中限制氧化应激来实现的。我们评估了MLF1（从树叶中提取）和AMF1（从树叶中提取）对醋酸铅（LA）诱导的大鼠毒性中脂质过氧化以及褪黑素、肿瘤坏死因子-α（TNF-α）和p53蛋白免疫调节的影响。
将60只成年雌性大鼠随机分为12组（每组n = 5）。第1组和第2组分别给予生理盐水和100 mg/kg体重的LA，持续5周。第3 - 6组给予100 mg/kg体重的LA，持续2周，随后分别用7.5和15 mg/kg体重的MLF1以及7.5和10 mg/kg体重的AMF1进行治疗，持续3周。第7 - 10组分别给予7.5和15 mg/kg体重的MLF1以及7.5和10 mg/kg体重的AMF1，持续5周。第11 - 12组分别给予100 mg/kg体重的LA与15 mg/kg体重的MLF1和10 mg/kg体重的AMF1联合给药，持续5周。药物和提取物通过口服给药。随后，评估肝脏组织病理学（苏木精和伊红染色）、血清褪黑素和TNF-α（酶联免疫吸附测定[ELISA]）水平。在肝脏匀浆中评估丙二醛（MDA）（硫代巴比妥酸测定法）和p53（ELISA）水平。对数据进行统计学分析（P≤0.05）。
所有组的肝脏组织学均显示正常。统计学分析表明，与第2组相比，第3 - 12组中MDA、TNF-α和p53水平有显著降低（P≤0.05）和不显著降低（P≥0.05）。此外，与第2组相比，第4 - 12组中褪黑素水平有显著升高（P≤0.05）和不显著升高（P≥0.05）。
本研究证实，MLF1和AMF1对LA诱导的大鼠毒性具有一定程度的抗氧化、抗癌和肝脏保护潜力。
