Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
STAR Protoc. 2021 Jul 7;2(3):100654. doi: 10.1016/j.xpro.2021.100654. eCollection 2021 Sep 17.
Quantitative assessment of neuropathological changes is essential for the characterization of animal models of neurodegenerative disease. Here, we describe a detailed protocol for the detection and quantification of key neuropathological changes in Alzheimer's mouse models. The protocol covers detailed methods including perfusion, dissection, and paraffinization of the brain, preparation of serial brain sections, immunohistochemical analysis, stereological quantification, and sample coding methods for genotype blind analysis. This protocol may be applied to the analysis of neuropathological changes of other neurological disorders. For complete details on the use and execution of this protocol, please refer to Lee et al. (2020), Kang and Shen (2020), Giaime et al. (2017), Xia et al. (2015), Watanabe et al. (2012, 2014), Wines-Samuelson et al. (2010), and Saura et al. (2004).
对神经病理学变化进行定量评估对于神经退行性疾病动物模型的特征化至关重要。在这里，我们描述了一种详细的方案，用于检测和量化阿尔茨海默病小鼠模型中的关键神经病理学变化。该方案涵盖了详细的方法，包括脑的灌注、解剖和石蜡包埋、脑的连续切片制备、免疫组织化学分析、体视学定量和用于基因型盲分析的样本编码方法。该方案可应用于其他神经紊乱的神经病理学变化分析。有关该方案使用和执行的完整详细信息，请参考 Lee 等人（2020 年）、Kang 和 Shen（2020 年）、Giaime 等人（2017 年）、Xia 等人（2015 年）、Watanabe 等人（2012 年、2014 年）、Wines-Samuelson 等人（2010 年）和 Saura 等人（2004 年）。
