Keizer H A, Beckers E, de Haan J, Janssen G M, Kuipers H, van Kranenburg G, Geurten P
Department of Physiology, University of Limburg.
Int J Sports Med. 1987 Dec;8 Suppl 3:151-3. doi: 10.1055/s-2008-1025721.
We have studied the effect of exercise on the percentage of free plasma estradiol 17-beta (E2) and testosterone (T) in 13 untrained (UT) and 8 trained (marathon runners, MR) eumenorrheic women. All women performed a standardized bicycle ergometer (UT) or treadmill (MR) test in the follicular and luteal phases of their menstrual cycles (15-min consecutive work loads of 60%, 70%, and 80% VO2 max to exhaustion). Blood was drawn through an indwelling venous catheter at 15 and 2 min before and immediately after exercise. Free E2 and T was assayed by centrifugal ultrafiltration using undiluted plasma at 37 degrees C (basal samples) and 39 degrees C (exercise samples). Statistical analysis was performed by a two-tailed paired t test. In the UT, the basal percentage of free E2 was measured to be 1.55% +/- 0.24% (mean +/- SD) in the follicular phase and 1.62% +/- 0.32% in the luteal phase (differences NS). In the MR, these values were 1.22% +/- 0.25% and 1.25% +/- 0.12% for the follicular and luteal phases, respectively. The differences between the groups were not significant. Exercise did not provoke significant changes in free E2 in either of the groups. Constrastingly, exercise induced a significant (P less than 0.02) increase in the percentage of free T from 1.56% +/- 0.27% to 2.1% +/- 0.36% and from 1.6% +/- 0.27% to 2.12% +/- 0.33% in the UT in the follicular and luteal phases, respectively. In the MR, the free percentage of T increased from 1.04% +/- 0.39% to 1.6% +/-0.16% (follicular phase) and from 1.24% +/- 0.22% to 1.67% +/- 0.18% (P less than 0.02).
我们研究了运动对13名未受过训练（UT）的和8名受过训练的（马拉松运动员，MR）月经周期正常的女性血浆中游离雌二醇17-β（E2）和睾酮（T）百分比的影响。所有女性在月经周期的卵泡期和黄体期进行了标准化的自行车测力计（UT组）或跑步机（MR组）测试（以60%、70%和80%最大摄氧量的连续15分钟工作量直至力竭）。在运动前15分钟和2分钟以及运动后立即通过留置静脉导管采血。游离E2和T在37℃（基础样本）和39℃（运动样本）下使用未稀释血浆通过离心超滤法进行测定。采用双侧配对t检验进行统计分析。在UT组中，卵泡期游离E2的基础百分比测得为1.55%±0.24%（均值±标准差），黄体期为1.62%±0.32%（差异无统计学意义）。在MR组中，卵泡期和黄体期的这些值分别为1.22%±0.25%和1.25%±0.12%。两组之间的差异不显著。运动在两组中均未引起游离E2的显著变化。相反，运动导致UT组卵泡期游离T百分比从1.56%±0.27%显著增加（P<0.02）至2.1%±0.36%，黄体期从1.6%±0.27%增加至2.12%±0.33%。在MR组中，卵泡期游离T百分比从1.04%±0.39%增加至1.6%±0.16%，黄体期从1.24%±0.22%增加至1.67%±0.18%（P<0.02）。
